Search
Etienne Weiss Université de Strasbourg, 67404, France, France,
1 protocol

László Tora Development and Stem cells, IGBMC/CNRS/INSERM/Univ. of Strasbourg
1 protocol

Sascha Conic Institut de Génétique et de Biologie Moléculaire et Cellulaire, 67404 Illkirch, France, France,
1 protocol

Dominique Desplancq
  • Université de Strasbourg, 67404, France, France,
  • 1 Author merit

Education

PhD, University of Leeds, England, 1993

Current position

Research Engineer, CNRS-UMR7242, University of Strasbourg, France

Publications

  1. Conic, S., Desplancq, D., Ferrand, A., Fischer, V., Heyer, V., Reina San Martin, B., Pontabry, J., Oulad-Abdelghani, M., Babu, N. K., Wright, G. D., Molina, N., Weiss, E. and Tora, L. (2018). Imaging of native transcription factors and histone phosphorylation at high resolution in live cells. J Cell Biol 217(4): 1537-1552. 
  2. Desplancq, D., Freund, G., Conic, S., Sibler, A. P., Didier, P., Stoessel, A., Oulad-Abdelghani, M., Vigneron, M., Wagner, J., Mely, Y., Chatton, B., Tora, L. and Weiss, E. (2016). Targeting the replisome with transduced monoclonal antibodies triggers lethal DNA replication stress in cancer cells. Exp Cell Res 342(2): 145-158. 
  3. Postupalenko, V., Desplancq, D., Orlov, I., Arntz, Y., Spehner, D., Mely, Y., Klaholz, B. P., Schultz, P., Weiss, E. and Zuber, G. (2015). Protein Delivery System Containing a Nickel-Immobilized Polymer for Multimerization of Affinity-Purified His-Tagged Proteins Enhances Cytosolic Transfer. Angew Chem Int Ed Engl 54(36): 10583-10586. 
  4. Postupalenko, V., Sibler, A. P., Desplancq, D., Nomine, Y., Spehner, D., Schultz, P., Weiss, E. and Zuber, G. (2014). Intracellular delivery of functionally active proteins using self-assembling pyridylthiourea-polyethylenimine. J Control Release 178: 86-94.
  5. Freund, G., Sibler, A. P., Desplancq, D., Oulad-Abdelghani, M., Vigneron, M., Gannon, J., Van Regenmortel, M. H. and Weiss, E. (2013). Targeting endogenous nuclear antigens by electrotransfer of monoclonal antibodies in living cells. MAbs 5(4): 518-522. 
  6. Rinaldi, A. S., Freund, G., Desplancq, D., Sibler, A. P., Baltzinger, M., Rochel, N., Mely, Y., Didier, P. and Weiss, E. (2013). The use of fluorescent intrabodies to detect endogenous gankyrin in living cancer cells. Exp Cell Res 319(6): 838-849.
  7. Desplancq, D., Rinaldi, A. S., Stoessel, A., Sibler, A. P., Busso, D., Oulad-Abdelghani, M., Van Regenmortel, M. H. and Weiss, E. (2011). Single-chain Fv fragment antibodies selected from an intrabody library as effective mono- or bivalent reagents for in vitro protein detection. J Immunol Methods 369(1-2): 42-50.
  8. Desplancq, D., Rinaldi, A. S., Horzer, H., Ho, Y., Nierengarten, H., Atkinson, R. A., Kieffer, B. and Weiss, E. (2008). Automated overexpression and isotopic labelling of biologically active oncoproteins in the cyanobacterium Anabaena sp. PCC 7120. Biotechnol Appl Biochem 51(Pt 1): 53-61. 
  9. Sibler, A. P., Baltzinger, M., Choulier, L., Desplancq, D. and Altschuh, D. (2008). SPR identification of mild elution conditions for affinity purification of E6 oncoprotein, using a multivariate experimental design. J Mol Recognit 21(1): 46-54.
  10. Oblak, M., Grdadolnik, S. G., Kotnik, M., Poterszman, A., Atkinson, R. A., Nierengarten, H., Desplancq, D., Moras, D. and Solmajer, T. (2006). Biophysical characterization of an indolinone inhibitor in the ATP-binding site of DNA gyrase. Biochem Biophys Res Commun 349(4): 1206-1213.
  11. Nomine, Y., Masson, M., Charbonnier, S., Zanier, K., Ristriani, T., Deryckere, F., Sibler, A. P., Desplancq, D., Atkinson, R. A., Weiss, E., Orfanoudakis, G., Kieffer, B. and Trave, G. (2006). Structural and functional analysis of E6 oncoprotein: insights in the molecular pathways of human papillomavirus-mediated pathogenesis. Mol Cell 21(5): 665-678.
  12. Desplancq, D., Bernard, C., Sibler, A. P., Kieffer, B., Miguet, L., Potier, N., Van Dorsselaer, A. and Weiss, E. (2005). Combining inducible protein overexpression with NMR-grade triple isotope labeling in the cyanobacterium Anabaena sp. PCC 7120. Biotechniques 39(3): 405-411.
1 Protocol published
Electroporation of Labeled Antibodies to Visualize Endogenous Proteins and Posttranslational Modifications in Living Metazoan Cell Types
Authors:  Sascha Conic, Dominique Desplancq, László Tora and Etienne Weiss, date: 11/05/2018, view: 133, Q&A: 0
The spatiotemporal localization of different intracellular factors in real-time and their detection in live cells are important parameters to understand dynamic protein-based processes. Therefore, there is a demand to perform live-cell imaging and ...