Etienne Weiss Université de Strasbourg, 67404, France, France,
1 protocol

László Tora Development and Stem cells, IGBMC/CNRS/INSERM/Univ. of Strasbourg
1 protocol

Sascha Conic Institut de Génétique et de Biologie Moléculaire et Cellulaire, 67404 Illkirch, France, France,
1 protocol

Dominique Desplancq
  • Université de Strasbourg, 67404, France, France,
Research focus
  • Cell biology
  • 1 Author merit


PhD, University of Leeds, England, 1993

Current position

Research Engineer, CNRS-UMR7242, University of Strasbourg, France


  1. Conic, S., Desplancq, D., Ferrand, A., Fischer, V., Heyer, V., Reina San Martin, B., Pontabry, J., Oulad-Abdelghani, M., Babu, N. K., Wright, G. D., Molina, N., Weiss, E. and Tora, L. (2018). Imaging of native transcription factors and histone phosphorylation at high resolution in live cells. J Cell Biol 217(4): 1537-1552. 
  2. Desplancq, D., Freund, G., Conic, S., Sibler, A. P., Didier, P., Stoessel, A., Oulad-Abdelghani, M., Vigneron, M., Wagner, J., Mely, Y., Chatton, B., Tora, L. and Weiss, E. (2016). Targeting the replisome with transduced monoclonal antibodies triggers lethal DNA replication stress in cancer cells. Exp Cell Res 342(2): 145-158. 
  3. Postupalenko, V., Desplancq, D., Orlov, I., Arntz, Y., Spehner, D., Mely, Y., Klaholz, B. P., Schultz, P., Weiss, E. and Zuber, G. (2015). Protein Delivery System Containing a Nickel-Immobilized Polymer for Multimerization of Affinity-Purified His-Tagged Proteins Enhances Cytosolic Transfer. Angew Chem Int Ed Engl 54(36): 10583-10586. 
  4. Postupalenko, V., Sibler, A. P., Desplancq, D., Nomine, Y., Spehner, D., Schultz, P., Weiss, E. and Zuber, G. (2014). Intracellular delivery of functionally active proteins using self-assembling pyridylthiourea-polyethylenimine. J Control Release 178: 86-94.
  5. Freund, G., Sibler, A. P., Desplancq, D., Oulad-Abdelghani, M., Vigneron, M., Gannon, J., Van Regenmortel, M. H. and Weiss, E. (2013). Targeting endogenous nuclear antigens by electrotransfer of monoclonal antibodies in living cells. MAbs 5(4): 518-522. 
  6. Rinaldi, A. S., Freund, G., Desplancq, D., Sibler, A. P., Baltzinger, M., Rochel, N., Mely, Y., Didier, P. and Weiss, E. (2013). The use of fluorescent intrabodies to detect endogenous gankyrin in living cancer cells. Exp Cell Res 319(6): 838-849.
  7. Desplancq, D., Rinaldi, A. S., Stoessel, A., Sibler, A. P., Busso, D., Oulad-Abdelghani, M., Van Regenmortel, M. H. and Weiss, E. (2011). Single-chain Fv fragment antibodies selected from an intrabody library as effective mono- or bivalent reagents for in vitro protein detection. J Immunol Methods 369(1-2): 42-50.
  8. Desplancq, D., Rinaldi, A. S., Horzer, H., Ho, Y., Nierengarten, H., Atkinson, R. A., Kieffer, B. and Weiss, E. (2008). Automated overexpression and isotopic labelling of biologically active oncoproteins in the cyanobacterium Anabaena sp. PCC 7120. Biotechnol Appl Biochem 51(Pt 1): 53-61. 
  9. Sibler, A. P., Baltzinger, M., Choulier, L., Desplancq, D. and Altschuh, D. (2008). SPR identification of mild elution conditions for affinity purification of E6 oncoprotein, using a multivariate experimental design. J Mol Recognit 21(1): 46-54.
  10. Oblak, M., Grdadolnik, S. G., Kotnik, M., Poterszman, A., Atkinson, R. A., Nierengarten, H., Desplancq, D., Moras, D. and Solmajer, T. (2006). Biophysical characterization of an indolinone inhibitor in the ATP-binding site of DNA gyrase. Biochem Biophys Res Commun 349(4): 1206-1213.
  11. Nomine, Y., Masson, M., Charbonnier, S., Zanier, K., Ristriani, T., Deryckere, F., Sibler, A. P., Desplancq, D., Atkinson, R. A., Weiss, E., Orfanoudakis, G., Kieffer, B. and Trave, G. (2006). Structural and functional analysis of E6 oncoprotein: insights in the molecular pathways of human papillomavirus-mediated pathogenesis. Mol Cell 21(5): 665-678.
  12. Desplancq, D., Bernard, C., Sibler, A. P., Kieffer, B., Miguet, L., Potier, N., Van Dorsselaer, A. and Weiss, E. (2005). Combining inducible protein overexpression with NMR-grade triple isotope labeling in the cyanobacterium Anabaena sp. PCC 7120. Biotechniques 39(3): 405-411.
1 Protocol published
Electroporation of Labeled Antibodies to Visualize Endogenous Proteins and Posttranslational Modifications in Living Metazoan Cell Types
Authors:  Sascha Conic, Dominique Desplancq, László Tora and Etienne Weiss, date: 11/05/2018, view: 2092, Q&A: 0
The spatiotemporal localization of different intracellular factors in real-time and their detection in live cells are important parameters to understand dynamic protein-based processes. Therefore, there is a demand to perform live-cell imaging and ...
We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.