László Tora Development and Stem cells, IGBMC/CNRS/INSERM/Univ. of Strasbourg
1 protocol

Dominique Desplancq Université de Strasbourg, 67404, France, France,
1 protocol

Sascha Conic Institut de Génétique et de Biologie Moléculaire et Cellulaire, 67404 Illkirch, France, France,
1 protocol

Etienne Weiss
  • Université de Strasbourg, 67404, France, France,
  • 1 Author merit

Education

PhD in Molecular and Cellular Biology, Université Louis Pasteur, France

Current position

Professor of Biotechnology, Ecole Supérieure de Biotechnologie, University of Strasbourg, France

Publications

  1. Desplancq, D., Groysbeck, N., Chiper, M., Weiss, E., Frisch, B., Strub, J.-M., Cianferani, S., Zafeiratos, S., Moeglin, E., Holy, X., Favier, A. L., De Carlo, S., Schultz, P., Spehner, D. and Zuber, G. (2018). Cytosolic Diffusion and Peptide-Assisted Nuclear Shuttling of Peptide-Substituted Circa 102 Gold Atom Nanoclusters in Living Cells. ACS Applied Nano Materials 1(8): 4236-4246. 
  2. Conic, S., Desplancq, D., Ferrand, A., Fischer, V., Heyer, V., Reina San Martin, B., Pontabry, J., Oulad-Abdelghani, M., Babu, N. K., Wright, G. D., Molina, N., Weiss, E. and Tora, L. (2018). Imaging of native transcription factors and histone phosphorylation at high resolution in live cells. J Cell Biol 217(4): 1537-1552.
  3. Dietsch, F., Donzeau, M., Cordonnier, A. M., Weiss, E., Chatton, B. and Vigneron, M. (2017). A fast method for analyzing essential protein mutants in human cells. Biotechniques 62(2): 80-82. 
  4. Freund, G., Vigneron, M., Nyamay'Antu, A., Desplancq, D. and Weiss, E. (2016). CRISPR/Cas9-mediated gene disruption using jetPRIME.
  5. Desplancq, D., Freund, G., Conic, S., Sibler, A. P., Didier, P., Stoessel, A., Oulad-Abdelghani, M., Vigneron, M., Wagner, J., Mely, Y., Chatton, B., Tora, L. and Weiss, E. (2016). Targeting the replisome with transduced monoclonal antibodies triggers lethal DNA replication stress in cancer cells. Exp Cell Res 342(2): 145-158.
  6. Postupalenko, V., Desplancq, D., Orlov, I., Arntz, Y., Spehner, D., Mely, Y., Klaholz, B. P., Schultz, P., Weiss, E. and Zuber, G. (2015). Protein Delivery System Containing a Nickel-Immobilized Polymer for Multimerization of Affinity-Purified His-Tagged Proteins Enhances Cytosolic Transfer. Angew Chem Int Ed Engl 54(36): 10583-10586. 
  7. Guilini, C., Baehr, C., Schaeffer, E., Gizzi, P., Rufi, F., Haiech, J., Weiss, E., Bonnet, D. and Galzi, J. L. (2015). New fluorescein precursors for live bacteria detection. Anal Chem 87(17): 8858-8866. 
  8. Orlov, I., Schertel, A., Zuber, G., Klaholz, B., Drillien, R., Weiss, E., Schultz, P. and Spehner, D. (2015). Live cell immunogold labelling of RNA polymerase II. Sci Rep 5: 8324.
  9. Robin, G., Sato, Y., Desplancq, D., Rochel, N., Weiss, E. and Martineau, P. (2014). Restricted diversity of antigen binding residues of antibodies revealed by computational alanine scanning of 227 antibody-antigen complexes. J Mol Biol 426(22): 3729-3743.
  10. Postupalenko, V., Sibler, A. P., Desplancq, D., Nomine, Y., Spehner, D., Schultz, P., Weiss, E. and Zuber, G. (2014). Intracellular delivery of functionally active proteins using self-assembling pyridylthiourea-polyethylenimine. J Control Release 178: 86-94.
  11. Freund, G., Desplancq, D., Stoessel, A., Weinsanto, R., Sibler, A. P., Robin, G., Martineau, P., Didier, P., Wagner, J. and Weiss, E. (2014). Generation of an intrabody-based reagent suitable for imaging endogenous proliferating cell nuclear antigen in living cancer cells. J Mol Recognit 27(9): 549-558.
  12. Freund, G., Sibler, A. P., Desplancq, D., Oulad-Abdelghani, M., Vigneron, M., Gannon, J., Van Regenmortel, M. H. and Weiss, E. (2013). Targeting endogenous nuclear antigens by electrotransfer of monoclonal antibodies in living cells. MAbs 5(4): 518-522.
  13. Rinaldi, A. S., Freund, G., Desplancq, D., Sibler, A. P., Baltzinger, M., Rochel, N., Mely, Y., Didier, P. and Weiss, E. (2013). The use of fluorescent intrabodies to detect endogenous gankyrin in living cancer cells. Exp Cell Res 319(6): 838-849.
  14. Desplancq, D., Rinaldi, A. S., Stoessel, A., Sibler, A. P., Busso, D., Oulad-Abdelghani, M., Van Regenmortel, M. H. and Weiss, E. (2011). Single-chain Fv fragment antibodies selected from an intrabody library as effective mono- or bivalent reagents for in vitro protein detection. J Immunol Methods 369(1-2): 42-50.
  15. Creusat, G., Rinaldi, A. S., Weiss, E., Elbaghdadi, R., Remy, J. S., Mulherkar, R. and Zuber, G. (2010). Proton sponge trick for pH-sensitive disassembly of polyethylenimine-based siRNA delivery systems. Bioconjug Chem 21(5): 994-1002.
  16. Chames, P., Van Regenmortel, M., Weiss, E. and Baty, D. (2009). Therapeutic antibodies: successes, limitations and hopes for the future. Br J Pharmacol 157(2): 220-233.
  17. Didier, P., Weiss, E., Sibler, A. P., Philibert, P., Martineau, P., Bigot, J. Y. and Guidoni, L. (2008). Femtosecond spectroscopy probes the folding quality of antibody fragments expressed as GFP fusions in the cytoplasm. Biochem Biophys Res Commun 366(4): 878-884.
  18. Desplancq, D., Rinaldi, A. S., Horzer, H., Ho, Y., Nierengarten, H., Atkinson, R. A., Kieffer, B. and Weiss, E. (2008). Automated overexpression and isotopic labelling of biologically active oncoproteins in the cyanobacterium Anabaena sp. PCC 7120. Biotechnol Appl Biochem 51(Pt 1): 53-61.
  19. Philibert, P., Stoessel, A., Wang, W., Sibler, A. P., Bec, N., Larroque, C., Saven, J. G., Courtete, J., Weiss, E. and Martineau, P. (2007). A focused antibody library for selecting scFvs expressed at high levels in the cytoplasm. BMC Biotechnol 7: 81.
  20. Lagrange, M., Boulade-Ladame, C., Mailly, L., Weiss, E., Orfanoudakis, G. and Deryckere, F. (2007). Intracellular scFvs against the viral E6 oncoprotein provoke apoptosis in human papillomavirus-positive cancer cells. Biochem Biophys Res Commun 361(2): 487-492.
  21. Courtete, J., Sibler, A. P., Zeder-Lutz, G., Dalkara, D., Oulad-Abdelghani, M., Zuber, G. and Weiss, E. (2007). Suppression of cervical carcinoma cell growth by intracytoplasmic codelivery of anti-oncoprotein E6 antibody and small interfering RNA. Mol Cancer Ther 6(6): 1728-1735.
  22. Nomine, Y., Masson, M., Charbonnier, S., Zanier, K., Ristriani, T., Deryckere, F., Sibler, A. P., Desplancq, D., Atkinson, R. A., Weiss, E., Orfanoudakis, G., Kieffer, B. and Trave, G. (2006). Structural and functional analysis of E6 oncoprotein: insights in the molecular pathways of human papillomavirus-mediated pathogenesis. Mol Cell 21(5): 665-678. 
  23. Desplancq, D., Bernard, C., Sibler, A. P., Kieffer, B., Miguet, L., Potier, N., Van Dorsselaer, A. and Weiss, E. (2005). Combining inducible protein overexpression with NMR-grade triple isotope labeling in the cyanobacterium Anabaena sp. PCC 7120. Biotechniques 39(3): 405-411.
  24. Sibler, A. P., Courtete, J., Muller, C. D., Zeder-Lutz, G. and Weiss, E. (2005). Extended half-life upon binding of destabilized intrabodies allows specific detection of antigen in mammalian cells. FEBS J 272(11): 2878-2891.
  25. Didier, P., Guidoni, L., Bigot, J. Y. and Weiss, E. (2005). Ultrafast gain dynamics of the green fluorescent protein fused with single-chain antibody Fv fragment. In "Ultrafast Phenomena XIV", Springer Series in Chemical Physics, 2005, Vol. 79. Edited by T. Kobayashi, T. Okada, T. Kobayashi, K. A. Nelson, S. De Silvestri. Springer, Berlin.
  26. Lagrange, M., Charbonnier, S., Orfanoudakis, G., Robinson, P., Zanier, K., Masson, M., Lutz, Y., Trave, G., Weiss, E. and Deryckere, F. (2005). Binding of human papillomavirus 16 E6 to p53 and E6AP is impaired by monoclonal antibodies directed against the second zinc-binding domain of E6. J Gen Virol 86(Pt 4): 1001-1007.
  27. Didier, P., Guidoni, L., Schwalbach, G., Weiss, E., Bourotte, M., Follénius-Wund, A., Pigault, C. and Bigot, J. Y. (2004). Ultrafast excited-state dynamics of the green fluorescent protein. In "Femtosecond Laser Applications in Biology", Avrillier, S. and Tualle, J. M. (Eds). SPIE Proc, USA. 5463, 118-128. 
  28. Weber, M., Weiss, E. and Engel, A. M. (2003). Combining EL4-B5-based B-cell stimulation and phage display technology for the successful isolation of human anti-Scl-70 autoantibody fragments. J Immunol Methods 278(1-2): 249-259.
  29. Masson, M., Hindelang, C., Sibler, A. P., Schwalbach, G., Trave, G. and Weiss, E. (2003). Preferential nuclear localization of the human papillomavirus type 16 E6 oncoprotein in cervical carcinoma cells. J Gen Virol 84(Pt 8): 2099-2104.
  30. Sibler, A. P., Nordhammer, A., Masson, M., Martineau, P., Trave, G. and Weiss, E. (2003). Nucleocytoplasmic shuttling of antigen in mammalian cells conferred by a soluble versus insoluble single-chain antibody fragment equipped with import/export signals. Exp Cell Res 286(2): 276-287.
  31. Nomine, Y., Charbonnier, S., Ristriani, T., Stier, G., Masson, M., Cavusoglu, N., Van Dorsselaer, A., Weiss, E., Kieffer, B. and Trave, G. (2003). Domain substructure of HPV E6 oncoprotein: biophysical characterization of the E6 C-terminal DNA-binding domain. Biochemistry 42(17): 4909-4917.
1 Protocol published
Electroporation of Labeled Antibodies to Visualize Endogenous Proteins and Posttranslational Modifications in Living Metazoan Cell Types
Authors:  Sascha Conic, Dominique Desplancq, László Tora and Etienne Weiss, date: 11/05/2018, view: 161, Q&A: 0
The spatiotemporal localization of different intracellular factors in real-time and their detection in live cells are important parameters to understand dynamic protein-based processes. Therefore, there is a demand to perform live-cell imaging and ...
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