Marc-David Ruepp Basic and Clinical Neuroscience, King's College London, UK, UK,
1 protocol

Jonas Mechtersheimer Basic and Clinical Neuroscience, King's College London, UK, UK,
1 protocol

Stefan Reber
  • Departement für Chemie und Biochemie, University of Bern, Switzerland, Switzerland,
  • 1 Author merit

Education

PhD, University of Bern, Switzerland, 2018

Current position

Research Associate, Department of Basic and Clinical Neuroscience, King’s College London, United Kingdom

Publications

  1. Hock, E. M., Maniecka, Z., Hruska-Plochan, M., Reber, S., Laferriere, F., Sahadevan, M. K. S., Ederle, H., Gittings, L., Pelkmans, L., Dupuis, L., Lashley, T., Ruepp, M. D., Dormann, D. and Polymenidou, M. (2018). Hypertonic Stress Causes Cytoplasmic Translocation of Neuronal, but Not Astrocytic, FUS due to Impaired Transportin Function. Cell Rep 24(4): 987-1000 e1007.
  2. Reber, S., Mechtersheimer, J., Nasif, S., Benitez, J. A., Colombo, M., Domanski, M., Jutzi, D., Hedlund, E. and Ruepp, M. D. (2018). CRISPR-Trap: a clean approach for the generation of gene knockouts and gene replacements in human cells. Mol Biol Cell 29(2): 75-83.
  3. Reber, S., Stettler, J., Filosa, G., Colombo, M., Jutzi, D., Lenzken, S. C., Schweingruber, C., Bruggmann, R., Bachi, A., Barabino, S. M., Muhlemann, O. and Ruepp, M. D. (2016). Minor intron splicing is regulated by FUS and affected by ALS-associated FUS mutants. EMBO J 35(14): 1504-1521.
  4. Suarez-Calvet, M., Neumann, M., Arzberger, T., Abou-Ajram, C., Funk, E., Hartmann, H., Edbauer, D., Kremmer, E., Gobl, C., Resch, M., Bourgeois, B., Madl, T., Reber, S., Jutzi, D., Ruepp, M. D., Mackenzie, I. R., Ansorge, O., Dormann, D. and Haass, C. (2016). Monomethylated and unmethylated FUS exhibit increased binding to Transportin and distinguish FTLD-FUS from ALS-FUS. Acta Neuropathol 131(4): 587-604.
  5. Raczynska, K. D., Ruepp, M. D., Brzek, A., Reber, S., Romeo, V., Rindlisbacher, B., Heller, M., Szweykowska-Kulinska, Z., Jarmolowski, A. and Schumperli, D. (2015). FUS/TLS contributes to replication-dependent histone gene expression by interaction with U7 snRNPs and histone-specific transcription factors. Nucleic Acids Res 43(20): 9711-9728.
1 Protocol published
Generation of Gene Knockout and Gene Replacement with Complete Removal of Full-length Endogenous Transcript Using CRISPR-Trap
Authors:  Jonas Mechtersheimer, Stefan Reber and Marc-David Ruepp, date: 10/20/2018, view: 478, Q&A: 0
This protocol describes the application of the CRISPR-Trap from designing of the gene targeting strategy to validation of successfully edited clones that was validated on various human cell lines, among them human induced pluripotent stem cells ...
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