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Philipp Schmidpeter Anesthesiology, Weill Cornell Medicine, USA, USA,
1 protocol

Crina M. Nimigean
  • Anesthesiology, Weill Cornell Medicine, USA, USA,
  • 1 Author merit

Education

Ph.D., University of Miami, USA, 1999

Current position

Associate Professor, Anesthesiology, Weill Cornell Medical College, USA

Publications (Since 2006)

  1. Marchesi A., Gao X., Adaixo R., Rheinberger J., Stahlberg H., Nimigean C.M.*, Scheuring S. (2018) An iris diaphragm mechanism to gate a cyclic nucleotide-gated ion channel. (Unpublished)
  2. Schmidpeter, P. A. M., Gao, X., Uphadyay, V., Rheinberger, J. and Nimigean, C. M. (2018). Ligand binding and activation properties of the purified bacterial cyclic nucleotide–gated channel SthK. The Journal of General Physiology

  3. Rheinberger, J., Gao, X., Schmidpeter, P. A. and Nimigean, C. M. (2018). Ligand discrimination and gating in cyclic nucleotide-gated ion channels from apo and partial agonist-bound cryo-EM structures. Elife 7. 

  4. Posson, D. J., Rusinova, R., Andersen, O. S. and Nimigean, C. M. (2018). Stopped-Flow Fluorometric Ion Flux Assay for Ligand-Gated Ion Channel Studies. Methods Mol Biol 1684: 223-235. 

  5. Kowal, J., Biyani, N., Chami, M., Scherer, S., Rzepiela, A. J., Baumgartner, P., Upadhyay, V., Nimigean, C. M. and Stahlberg, H. (2018). High-Resolution Cryoelectron Microscopy Structure of the Cyclic Nucleotide-Modulated Potassium Channel MloK1 in a Lipid Bilayer. Structure 26(1): 20-27.e23. 

  6. Menny, A., Lefebvre, S. N., Schmidpeter, P. A., Drege, E., Fourati, Z., Delarue, M., Edelstein, S. J., Nimigean, C. M., Joseph, D. and Corringer, P. J. (2017). Identification of a pre-active conformation of a pentameric channel receptor. Elife 6. 

  7. Heer, F. T., Posson, D. J., Wojtas-Niziurski, W., Nimigean, C. M. and Berneche, S. (2017). Mechanism of activation at the selectivity filter of the KcsA K(+) channel. Elife 6. 

  8. Rangl, M., Miyagi, A., Kowal, J., Stahlberg, H., Nimigean, C. M. and Scheuring, S. (2016). Real-time visualization of conformational changes within single MloK1 cyclic nucleotide-modulated channels. Nature Communications 7: 12789. 

  9. Kim, D. M. and Nimigean, C. M. (2016). Voltage-Gated Potassium Channels: A Structural Examination of Selectivity and Gating. Cold Spring Harb Perspect Biol 8(5). 

  10. Kim, D. M., Dikiy, I., Upadhyay, V., Posson, D. J., Eliezer, D. and Nimigean, C. M. (2016). Conformational heterogeneity in closed and open states of the KcsA potassium channel in lipid bicelles. J Gen Physiol 148(2): 119-132. 

  11. Posson, D. J., Rusinova, R., Andersen, O. S. and Nimigean, C. M. (2015). Calcium ions open a selectivity filter gate during activation of the MthK potassium channel. Nat Commun 6: 8342. 

  12. Kim D.M., McCoy J.G., and Nimigean C.M. (2014) Ion Selectivity and Conductance. chapter 2 in Handbook of Ion Channels,  Taylor and Francis group, CRC Press.

  13. Rusinova, R., Kim, D. M., Nimigean, C. M. and Andersen, O. S. (2014). Regulation of ion channel function by the host lipid bilayer examined by a stopped-flow spectrofluorometric assay. Biophys J 106(5): 1070-1078. 

  14. McCoy, J. G., Rusinova, R., Kim, D. M., Kowal, J., Banerjee, S., Jaramillo Cartagena, A., Thompson, A. N., Kolmakova-Partensky, L., Stahlberg, H., Andersen, O. S. and Nimigean, C. M. (2014). A KcsA/MloK1 chimeric ion channel has lipid-dependent ligand-binding energetics. J Biol Chem 289(14): 9535-9546. 

  15. Kowal, J., Chami, M., Baumgartner, P., Arheit, M., Chiu, P. L., Rangl, M., Scheuring, S., Schroder, G. F., Nimigean, C. M. and Stahlberg, H. (2014). Ligand-induced structural changes in the cyclic nucleotide-modulated potassium channel MloK1. Nat Commun 5: 3106. 

  16. Thompson, A. N. and Nimigean, C. M. (2013). Potassium Channels, Structure and Function. Encyclopedia of Metalloproteins. R. H. Kretsinger, V. N. Uversky and E. A. Permyakov. New York, NY, Springer New York: 1790-1794.

  17. Thompson, A. N. and Nimigean, C. M. (2013). Potassium Channel Diversity, Regulation of Potassium Flux across Pores. Encyclopedia of Metalloproteins. R. H. Kretsinger, V. N. Uversky and E. A. Permyakov. New York, NY, Springer New York: 1781-1785.

  18. Posson, D. J., Thompson, A. N., McCoy, J. G. and Nimigean, C. M. (2013). Molecular interactions involved in proton-dependent gating in KcsA potassium channels. J Gen Physiol 142(6): 613-624. 

  19. Posson, D. J., McCoy, J. G. and Nimigean, C. M. (2013). The voltage-dependent gate in MthK potassium channels is located at the selectivity filter. Nat Struct Mol Biol 20(2): 159-166. 

  20. Bhate, M. P., Wylie, B. J., Thompson, A., Tian, L., Nimigean, C. and McDermott, A. E. (2013). Preparation of uniformly isotope labeled KcsA for solid state NMR: expression, purification, reconstitution into liposomes and functional assay. Protein Expr Purif 91(2): 119-124. 

  21. McCoy, J. G. and Nimigean, C. M. (2012). Structural correlates of selectivity and inactivation in potassium channels. Biochim Biophys Acta 1818(2): 272-285. 

  22. Nimigean, C. M. and Allen, T. W. (2011). Origins of ion selectivity in potassium channels from the perspective of channel block. J Gen Physiol 137(5): 405-413. 

  23. Cheng, W. W., McCoy, J. G., Thompson, A. N., Nichols, C. G. and Nimigean, C. M. (2011). Mechanism for selectivity-inactivation coupling in KcsA potassium channels. Proc Natl Acad Sci U S A 108(13): 5272-5277. 

  24. Banerjee, S. and Nimigean, C. M. (2011). Non-vesicular transfer of membrane proteins from nanoparticles to lipid bilayers. J Gen Physiol 137(2): 217-223. 

  25. Tanabe, M., Nimigean, C. M. and Iverson, T. M. (2010). Structural basis for solute transport, nucleotide regulation, and immunological recognition of Neisseria meningitidis PorB. Proc Natl Acad Sci U S A 107(15): 6811-6816. 

  26. Thompson, A. N., Kim, I., Panosian, T. D., Iverson, T. M., Allen, T. W. and Nimigean, C. M. (2009). Mechanism of potassium-channel selectivity revealed by Na(+) and Li(+) binding sites within the KcsA pore. Nat Struct Mol Biol 16(12): 1317-1324. 

  27. Thompson, A. N., Posson, D. J., Parsa, P. V. and Nimigean, C. M. (2008). Molecular mechanism of pH sensing in KcsA potassium channels. Proc Natl Acad Sci U S A 105(19): 6900-6905. 

  28. Nimigean, C. M. and Pagel, M. D. (2007). Ligand binding and activation in a prokaryotic cyclic nucleotide-modulated channel. J Mol Biol 371(5): 1325-1337. 

  29. Chiu, P. L., Pagel, M. D., Evans, J., Chou, H. T., Zeng, X., Gipson, B., Stahlberg, H. and Nimigean, C. M. (2007). The structure of the prokaryotic cyclic nucleotide-modulated potassium channel MloK1 at 16 A resolution. Structure 15(9): 1053-1064. 

  30. Zadek, B. and Nimigean, C. M. (2006). Calcium-dependent gating of MthK, a prokaryotic potassium channel. J Gen Physiol 127(6): 673-685. 

  31. Nimigean, C. M. (2006). A radioactive uptake assay to measure ion transport across ion channel-containing liposomes. Nat Protoc 1(3): 1207-1212.

1 Protocol published
Fluorescence Titrations to Determine the Binding Affinity of Cyclic Nucleotides to SthK Ion Channels
Authors:  Philipp A.M. Schmidpeter and Crina M. Nimigean, date: 10/05/2018, view: 199, Q&A: 0
The cyclic-nucleotide modulated ion channel family includes cyclic nucleotide-gated (CNG) and hyperpolarization-activated and cyclic nucleotide-modulated (HCN) channels, which play essential roles in visual and olfactory signaling and the heart ...