Julián Gómez-Gutiérrez Biochemistry and Molecular Biology, University Complutense , Spain, Spain,
2 protocols

Mar Rodríguez-Rodríguez Hospital Universitario Ramón y Cajal, Spain
2 protocols

Belén Yélamos Biochemistry and Molecular Biology, University Complutense , Spain, Spain,
2 protocols

Francisco Gavilanes
  • Biochemistry and Molecular Biology, University Complutense , Spain, Spain,
  • 2 Author merit
2 Protocols published
In vitro Membrane Interaction and Liposome Fusion Assays Using Recombinant Hepatitis C Virus Envelope Protein E2
In order to study the mechanism underlying the Hepatitis C Virus (HCV) fusion process we have performed assays using phospholipid liposomes and a truncated form of E2 protein, E2661 (amino acids 384-661 of the HCV polyprotein) lacking the ...
Expression and Ni-NTA-Agarose Purification of Recombinant Hepatitis C Virus E2 Ectodomain Produced in a Baculovirus Expression System
In this protocol, we describe the production and purification of the ectodomain of the E2661 envelope protein (amino acids 384-661) of the Hepatitis C virus, which plays a fundamental role in the entry of the virus into the host cell. ...
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