Matej Hotka Department of Pharmacology, Medical University of Vienna, Austria, Austria,
1 protocol

Verena Burtscher Institute of Pharmacology and the Gaston H. Glock Research Laboratories for Exploratory Drug Development, Center of Physiology and Pharmacology, Medical University of Vienna, Austria, Austria,
1 protocol

Walter Sandtner
  • Department of Pharmacology, Medical University of Vienna, Austria, Austria,
Research focus
  • Biophysics
  • 1 Author merit


Dr, Universität für Bodenkultur, Austria, 2004

Current position

Ass.Prof, Pharmacology, Medical University of Vienna, Austria

Publications (§ corresponding author, * contributed equally)

  1. Saha K, Li Y, Holy M, Lehner KR, Bukhari MO, Partilla JS, Sandtner W, Sitte HH, Baumann MH (2018). The synthetic cathinones, butylone and pentylone, are stimulants that act as dopamine transporter blockers but 5-HT transporter substrates. Psychopharmacology. [Epub ahead of print]

  2. Burtscher V, Hotka M, Li Y, Freissmuth M, Sandtner W§ (2018). A label-free approach to detect ligand binding to cell surface proteins in real time. Elife. 7.pii: e34944

  3. Hasenhuetl PS, Bhat S, Mayer FP, Sitte HH, Freissmuth M, Sandtner W§ (2018). A kinetic account for amphetamine-induced monoamine release. J Gen Physiol. [Epub ahead of print]

  4. Cervenka R, Lukacs P, Gawali VS, Ke S, Koenig X, Rubi L, Zarrabi T, Hilber K, Sandtner W, Stary-Weinzinger A, Todt H (2018). Distinct modulation of inactivation by a residue in the pore domain of voltage-gated Na+ channels: mechanistic insights from recent crystal structures. Sci.Rep. 8(1): 631

  5. Mayer FP, Burchardt NV, Decker AM, Partilla JS, Li Y, McLaughlin G, Kavanagh PV, Sandtner W, Blough BE, Brandt SD, Baumann MH, Sitte HH (2017). Fluorinated phenmetrazine "legal highs" act as substrates for high- affinity monoamine transporters of the SLC6 family. j.neuropharm. [Epub ahead of print]

  6. Bhat S, Hasenhuetl PS, Kasture A, El-Kasaby A, Baumann MH, Blough BE, Sucic S, Sandtner W, Freissmuth M (2017). Conformational state interactions provide clues to the pharmacochaperone potential of serotonin transporter partial substrates. J Biol Chem 292: 16773-16786

  7. Kern C, Erdem FA, El-Kasaby A, Sandtner W, Freissmuth M, Sucic S (2017). The N-Terminus specifies the switch between transport modes of the human Serotonin transporter. J Biol Chem 292: 3603-361

  8. Li Y, Mayer FP, Hasenhuetl PS, Burtscher V, Schicker K, Sitte HH, Freissmuth M, Sandtner W§ (2017). Occupancy of the Zinc Binding-site by Transition Metals decreases the Substrate Affinity of the Human Dopamine Transporter by an Allosteric Mechanism. J Biol Chem 292: 4235-4243

  9. Sohail A, Jayaraman K, Venkatesan S, Gotfryd K, Daerr M, Gether U, Loland CJ, Wanner KT, Freissmuth M, Sitte HH, Sandtner W, Stockner T (2016). The Environment Shapes the Inner Vestibule of LeuT. PloS Comput Biol 12: e1005197

  10. Hasenhuetl PS, Freissmuth M, Sandtner W§ (2016). Electrogenic Binding of Intracellular Cations Defines a Kinetic Decision Point in the Transport Cycle of the Human Serotonin Transporter. J Biol Chem 291: 25864-25876

  11. Zhu R, Sinwel D, Hasenhuetl PS, Saha K, Kumar V, Zhang P, Rankl C, Holy M, Sucic S, Kudlacek O, Karner A, Sandtner W, Stockner T, Gruber HJ, Freissmuth M, Hauck Newman A, Sitte HH, Hinterdorfer P (2016). Nanopharmacological Force Sensing to Reveal Allosteric Coupling in Transporter Binding Sites. Angew Chem Int Ed Eng 55: 1719-1722

  12. Li Y, Hasenhuetl PS, Schicker K, Sitte HH, Freissmuth M, Sandtner W§ (2015). Dual Action of Zn2+ on the Transport Cycle of the Dopamine Transporter. J Biol Chem 290: 31069-31076

  13. Sandtner W*, Stockner T*, Hasenhuetl PS, Partilla JS, Seddik A, Zhang YW, Cao J, Holy M,Steinkellner T, Rudnick G, Baumann MH, Ecker GF, Newman AH, Sitte HH (2016). Binding Mode Selection Determines the Action of Ecstasy Homologs at Monoamine Transporters. Mol Pharmacol 89: 165-175

  14. Venkatesan S, Saha K, Sohail A, Sandtner W, Freissmuth M, Ecker GF, Sitte HH, Stockner T (2015). Refinement of the Central Steps of Substrate Transport by the AspartateTransporter GltPh: Elucidating the Role of the Na2 Sodium Binding Site. PLoS Comput Biology 11: e1004551.

  15. Gawali VS, Lukacs P, Cervenka R, Koenig X, Rubi L, Hilber K, Sandtner W, Todt H (2015). Mechanism of Modification, by Lidocaine, of Fast and Slow Recovery from Inactivation of Voltage-Gated Na⁺ Channels. Mol Pharmacol 88: 866-879

  16. Hasenhuetl PS, Schicker K, Koenig X, Li Y, Sarker S, Stockner T, Sucic S, Sitte HH, Freissmuth M, Sandtner W§ (2015). Ligand Selectivity among the Dopamine and Serotonin Transporters Specified by the Forward Binding Reaction. Mol Pharmacol 88: 12-18

  17. Saha K, Partilla JS, Lehner KR, Seddik A, Stockner T, Holy M, Sandtner W, Ecker GF, Sitte HH, Baumann MH (2015). Second-generation' mephedrone analogs, 4-MEC and 4-MePPP, differentially affect monoamine transporter function. Neurophsychopharmacology 40: 1321-1331

  18. Schmid D, Koenig X, Bulusu S, Schicker K, Freissmuth M, Sitte HH, Sandtner W§ (2014). The conservative view: is it necessary o implant a stent into the dopamine transporter. Br J Pharmacol 172: 4775-4778

  19. Dalmas O, Sandtner W, Medovoy D, Frezza L, Bezanilla F, Perozo E (2014). A repulsion mechanism explains magnesium permeation and selectivity in CorA. Proc Natl Acad Sci U S A 111: 3002-3007

  20. Thurner P, Stary-Weinzinger A, Gafar H, Gawali VS, Kudlacek O, Zezula J, Hilber K, Boehm S, Sandtner W, Koenig X (2014). Mechanism of hERG channel block by the psychoactive indole alkaloid ibogaine. J Pharmacol Exp Ther 348: 346-358

  21. Felts B*, Pramod AB*, Sandtner W*, Burbach N, Bulling S, Sitte HH, Henry LK (2014). The two Na+ sites in the human serotonin transporter play distinct roles in the ion coupling and electrogenicity of transport. J Biol Chem 289: 1825-1840

  22. Baumann MH, Bulling S, Benaderet TS, Saha K, Ayestas MA, Partilla JS, Ali SF, Stockner T, Rothman RB, Sandtner W, Sitte HH (2014). Evidence for a Role of Transporter-Mediated Currents in the Depletion of Brain Serotonin Induced by Serotonin Transporter Substrates. Neuropsychopharmacology 39: 1355-1365

  23. Sandtner W*§, Schmid D*, Schicker K*, Gerstbrein K, Koenig X, Mayer FP, Boehm S, Freissmuth M, Sitte HH (2014). A quantitative model of amphetamine action on the 5-HT transporter. Br J Pharmacol 171: 1007-1018

  24. Villalba-Galea CA, Frezza L, Sandtner W, Bezanilla F (2013). Sensing charges of the Ciona intestinalis voltage-sensing phosphatase. J Gen Physiol 142: 543-555

  25. Koenig X, Kovar M, Rubi L, Mike AK, Lukacs P, Gawali VS, Todt H, Hilber K, Sandtner W (2013). Anti-addiction drug ibogaine inhibits voltage-gated ionic currents: a study to assess the drug's cardiac ion channel profile. Toxicol Appl Pharmacol 273: 259-268

  26. Hyde HC, Sandtner W, Vargas E, Dagcan AT, Robertson JL, Roux B, Correa AM, Bezanilla F (2012). Nano-positioning system for structural analysis of functional homomeric proteins in multiple conformations. Structure 20: 1629- 1640

  27. Koenig X, Kovar M, Boehm S, Sandtner W, Hilber K (2012). Anti-addiction drug ibogaine inhibits hERG channels: a cardiac arrhythmia risk. Addict Biol 19: 237-239

  28. Bulling S, Schicker K, Zhang YW, Steinkellner T, Stockner T, Gruber CW, Boehm S, Freissmuth M, Rudnick G, Sitte HH, Sandtner W§ (2012). The mechanistic basis for noncompetitive ibogaine inhibition of serotonin and dopamine transporters. J Biol Chem 287: 18524-18534

  29. Schicker K, Uzelac Z, Gesmonde J, Bulling S, Stockner T, Freissmuth M, Boehm S, Rudnick G, Sitte HH, Sandtner W§ (2012). Unifying concept of serotonin transporter-associated currents. J Biol Chem 287: 438-445

  30. Sandtner W, Egwolf B, Khalili-Araghi F, Sánchez-Rodríguez JE, Roux B, Bezanilla F, Holmgren M (2011). Ouabain binding site in a functioning Na+/K+ ATPase. J Biol Chem 286: 38177-38183

  31. Zarrabi T, Cervenka R, Sandtner W, Lukacs P, Koenig X, Hilber K, Mille M, Lipkind GM, Fozzard HA, Todt H (2010). A Molecular Switch between the Outer and the Inner Vestibules of the Voltage-gated Na+ Channel. J Biol Chem 285: 39458-39470

  32. Villalba-Galea CA, Sandtner W, Dimitrov D, Mutoh H, Knöpfel T, Bezanilla F (2009). Charge movement of a voltage-sensitive fluorescent protein. Biophys J 96: L19-21

  33. Villalba-Galea CA, Sandtner W, Starace D, Bezanilla F (2008). S4-based Voltage Sensors have three major conformations. Proc Natl Acad Sci U S A 105: 17600-17607

  34. Sandtner W, Bezanilla F, Correa AM (2007). In vivo measurements of intramolecular distances using genetically encoded reporters. Biophys J 93: L45-47

  35. Szendrödi J*, Sandtner W*, Zarrabi T, Zebedin E, Hilber K, Dudley SC, Fozzard HA, Hannes Todt H (2007). Speeding the recovery from ultra-slow inactivation of voltage-gated Na+ channels by metal ion binding to the selectivity filter: a foot-on-the-door? Biophys J 93: 4209-4224

  36. Zebedin E, Mille M, Speiser M, Zarrabi T, Sandtner W, Latzenhofer B, Todt H, Hilber K (2007). C2C12 skeletal muscle cells adopt cardiac-like sodium current properties in a cardiac cell environment. Am J Physiol Heart Circ Physiol 292: H439-450

  37. Hilber K*, Sandtner W*, Zarrabi T, Zebedin E, Kudlacek O, Fozzard HA, Todt
    H. (2005). Selectivity filter residues contribute unequally to pore stabilization in voltage-gated sodium channels. Biochemistry 44: 13874-13882

  38. Hilber B, Scholze P, Dorostkar MM, Sandtner W, Holy M, Boehm S, Singer EA, Sitte HH (2005). Serotonin-transporter mediated efflux: A pharmacological analysis of amphetamines and non-amphetamines. Neuropharmacology 49: 811-819

  39. Zebedin E, Sandtner W, Galler S, Szendroedi J, Just H, Todt H, Hilber K (2004). Fiber type conversion alters inactivation of voltage-dependent sodium currents in mouse C2C12 skeletal muscle cells. Am J Physiol: Cell Physiol 287: C270-280

  40. Sandtner W*, Szendroedi J*, Zebedin E, Zarrabi T, Hilber K, Glaaser I, Fozzard HA, Dudley SC, Todt, H (2004). Lidocaine: A Foot in the Door of the Inner Vestibule of the Voltage-Gated Sodium Channel. Mol Pharmacol 66: 648-657

  41. Hilber K*, Sandtner W*, Kudlacek O, Schreiner B, Glaaser I, Schuetz W, Fozzard HA, Dudley SC, Todt H (2002). Interaction Between Fast and Ultra-Slow Inactivation in the Voltage-Gated Sodium channel: Does the Inactivation Gate Stabilize the Channel Structure? J Biol Chem 277: 37105-37115

  42. Hilber K, Sandtner W, Kudlacek O, Glaaser IW, Weisz E, Kyle JW, French RJ, Fozzard HA, Dudley SC, Todt H (2001). The selectivity filter of the voltage- gated sodium channel is involved in channel activation. J Biol Chem 276: 27831-27839

1 Protocol published
Authors:  Verena Burtscher, Matej Hotka and Walter Sandtner, date: 01/05/2019, view: 4059, Q&A: 0
In multi-cellular organisms, cells communicate with each other utilizing chemical messengers. For many of these messenger molecules, the membrane is an insurmountable barrier. Yet, they act by binding to surface proteins often triggering a cascade ...
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