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William M. Gelbart
  • Chemistry and Biochemistry, University of California, Los Angeles, USA, USA,
  • 1 Author merit

Education

PhD, University of Chicago, USA

Current position

Distinguished Professor, Chemistry and Biochemistry, UCLA, USA

Publications (Since 2011)

  1. Chung,  S., Lerner, E., Jin, Y., Kim, S., Alhadid, Y., Grimaud, L. W., Zhang, I. X., Knobler,  C. M., Gelbart, W. M. and Weiss, S(2018). The effect of macromolecular crowding on single-round transcription by E. coli RNA polymerase. (Unpublished).

  2. Garmann, R. F., Knobler, C. M. and Gelbart, W. M. (2018). Protocol for Efficient Cell-Free Synthesis of Cowpea Chlorotic Mottle Virus-Like Particles Containing Heterologous RNAs. Methods Mol Biol 1776: 249-265. 

  3. Chen, Y.-J., Wu, D., Gelbart, W., Knobler, C. M., Phillips, R. and Kegel, W. K. (2018). Two-Stage Dynamics of In Vivo Bacteriophage Genome Ejection. Physical Review X 8(2): 021029. 

  4. Beren,C.,  Liu, K. N .,Dreesens, L. L., Knobler, C. M. and Gelbart, W. M., (2018). PolyU synthesis and fractionation. Bio-protocol.

  5. Beren, C., Dreesens, L. L., Liu, K. N., Knobler, C. M. and Gelbart, W. M. (2017). The Effect of RNA Secondary Structure on the Self-Assembly of Viral Capsids. Biophys J 113(2): 339-347. 

  6. Biddlecome, A., Habte, H. H., McGrath, K. M., Sambanthamoorthy, S., Wurm, M., Knobler, C. M., Lorenz, I., Leppard, J. B., Lasaro, M., Elbers, K. and Gelbart, W. M  (2018). Delivery of SelfAmplifying RNA Vaccines in In Vitro Reconstituted Virus-Like Particles. (Unpublished)

  7. Garmann, R. F., Comas-Garcia, M., Knobler, C. M. and Gelbart, W. M. (2016). Physical Principles in the Self-Assembly of a Simple Spherical Virus. Acc Chem Res 49(1): 48-55. 

  8. Borodavka, A., Singaram, S. W., Stockley, P. G., Gelbart, W. M., Ben-Shaul, A. and Tuma, R. (2016). Sizes of Long RNA Molecules Are Determined by the Branching Patterns of Their Secondary Structures. Biophys J 111(10): 2077-2085. 

  9. Singaram, S. W., Garmann, R. F., Knobler, C. M., Gelbart, W. M. and Ben-Shaul, A. (2015). Role of RNA Branchedness in the Competition for Viral Capsid Proteins. J Phys Chem B 119(44): 13991-14002. 

  10. Jin, Y., Sdao, S. M., Dover, J. A., Porcek, N. B., Knobler, C. M., Gelbart, W. M. and Parent, K. N. (2015). Bacteriophage P22 ejects all of its internal proteins before its genome. Virology 485: 128-134. 

  11. Jin, Y., Knobler, C. M. and Gelbart, W. M. (2015). Controlling the extent of viral genome release by a combination of osmotic stress and polyvalent cations. Phys Rev E Stat Nonlin Soft Matter Phys 92(2): 022708. 

  12. Gelbart, W. M. (2015). DNA-peptide complexes: Regulation of interferon production. Nat Mater 14(7): 661-662. 

  13. Garmann, R. F., Sportsman, R., Beren, C., Manoharan, V. N., Knobler, C. M. and Gelbart, W. M. (2015). A Simple RNA-DNA Scaffold Templates the Assembly of Monofunctional Virus-Like Particles. J Am Chem Soc 137(24): 7584-7587. 

  14. Garmann, R. F., Gopal, A., Athavale, S. S., Knobler, C. M., Gelbart, W. M. and Harvey, S. C. (2015). Visualizing the global secondary structure of a viral RNA genome with cryo-electron microscopy. RNA 21(5): 877-886. 

  15. Ben-Shaul, A. and Gelbart, W. M. (2015). Viral ssRNAs are indeed compact. Biophys J 108(1): 14-16. 

  16. Vega-Acosta, J. R., Cadena-Nava, R. D., Gelbart, W. M., Knobler, C. M. and Ruiz-Garcia, J. (2014). Electrophoretic mobilities of a viral capsid, its capsid protein, and their relation to viral assembly. J Phys Chem B 118(8): 1984-1989.

  17. Gopal, A., Egecioglu, D. E., Yoffe, A. M., Ben-Shaul, A., Rao, A. L., Knobler, C. M. and Gelbart, W. M. (2014). Viral RNAs are unusually compact. PLoS One 9(9): e105875. 

  18. Garmann, R. F., Comas-Garcia, M., Koay, M. S., Cornelissen, J. J., Knobler, C. M. and Gelbart, W. M. (2014). Role of electrostatics in the assembly pathway of a single-stranded RNA virus. J Virol 88(18): 10472-10479. 

  19. Garmann, R. F., Comas-Garcia, M., Gopal, A., Knobler, C. M. and Gelbart, W. M. (2014). The assembly pathway of an icosahedral single-stranded RNA virus depends on the strength of inter-subunit attractions. J Mol Biol 426(5): 1050-1060. 

  20. Comas-Garcia, M., Garmann, R. F., Singaram, S. W., Ben-Shaul, A., Knobler, C. M. and Gelbart, W. M. (2014). Characterization of Viral Capsid Protein Self-Assembly around Short Single-Stranded RNA. J Phys Chem B 118(27): 7510-7519. 

  21. Azizgolshani, O., Garmann, R. F., Cadena-Nava, R., Knobler, C. M. and Gelbart, W. M. (2013). Reconstituted plant viral capsids can release genes to mammalian cells. Virology 441(1): 12-17. 

  22. Gopal, A., Zhou, Z. H., Knobler, C. M. and Gelbart, W. M. (2012). Visualizing large RNA molecules in solution. RNA 18(2): 284-299. 

  23. Comas-Garcia, M., Cadena-Nava, R. D., Rao, A. L., Knobler, C. M. and Gelbart, W. M. (2012). In vitro quantification of the relative packaging efficiencies of single-stranded RNA molecules by viral capsid protein. J Virol 86(22): 12271-12282. 

  24. Cadena-Nava, R. D., Comas-Garcia, M., Garmann, R. F., Rao, A. L., Knobler, C. M. and Gelbart, W. M. (2012). Self-assembly of viral capsid protein and RNA molecules of different sizes: requirement for a specific high protein/RNA mass ratio. J Virol 86(6): 3318-3326. 

  25. Yoffe, A. M., Prinsen, P., Gelbart, W. M. and Ben-Shaul, A. (2011). The ends of a large RNA molecule are necessarily close. Nucleic Acids Res 39(1): 292-299.

  26. Snyder, J. E., Azizgolshani, O., Wu, B., He, Y., Lee, A. C., Jose, J., Suter, D. M., Knobler, C. M., Gelbart, W. M. and Kuhn, R. J. (2011). Rescue of infectious particles from preassembled alphavirus nucleocapsid cores. J Virol 85(12): 5773-5781. 

  27. Qiu, X., Rau, D. C., Parsegian, V. A., Fang, L. T., Knobler, C. M. and Gelbart, W. M. (2011). Salt-dependent DNA-DNA spacings in intact bacteriophage lambda reflect relative importance of DNA self-repulsion and bending energies. Phys Rev Lett 106(2): 028102. 

  28. Fang, L. T., Yoffe, A. M., Gelbart, W. M. and Ben-Shaul, A. (2011). A sequential folding model predicts length-independent secondary structure properties of long ssRNA. J Phys Chem B 115(12): 3193-3199. 

  29. Fang, L. T., Gelbart, W. M. and Ben-Shaul, A. (2011). The size of RNA as an ideal branched polymer. J Chem Phys 135(15): 155105. 

  30. Cadena-Nava, R. D., Hu, Y., Garmann, R. F., Ng, B., Zelikin, A. N., Knobler, C. M. and Gelbart, W. M. (2011). Exploiting fluorescent polymers to probe the self-assembly of virus-like particles. J Phys Chem B 115(10): 2386-2391.


1 Protocol published
Enzymatic Synthesis and Fractionation of Fluorescent PolyU RNAs
The physical properties of viral-length polyuridine (PolyU) RNAs, which cannot base-pair and form secondary structures, are compared with those of normal-composition RNAs, composed of comparable numbers of each of A, U, G and C nucleobases. In this ...