Good afternoon, I am trying to make 18 % Acrylamide gel following your protocol. Problem is 30 µl Teamed for 10 ml separating gel is quite high, because of it immediately going to polymerized in the tube. So, can I decreased teamed amount? My another question is, why you used 5 M (3 g) urea? Without urea, there is any problem of protein migration in the gel? My protein concentration is low. Therefore, could I load 50µl protein in the gel?
Thank you.
7/2/2020 10:34:42 PM Reply