Hello, thank you for your protocol. I'm interested in peptide 2 kDa. I have tried your protocol: 18% glycine - SDS- PAGE, 25 A for 3,5 hours. I use ruler 10- 250 kDa. The green band is 10kDa. And this is the results. Nearby the green band is 2 lanes of bromophenol (0,6 kDa as you wrote). I have 2 question:
- How can i reduce the time, because 3,5 hours is too long,
- How can i separate the geen band and bromophenol so that i can sure that i can detect peptide 2kDa between them.
Can i make 25% gel and put more ure??? Please reply me!!!! Thanks a lot
10/20/2020 6:48:52 AM Reply