Hi, Good Afternoon, I have some question about this protocol.

This protocol mention that, After sample load in 18% polyacrylamide gel, run time should keep 45 min under constant 25 A, but according this time sample just inter into the separating gel. So my question is after 45 min, how long I will keep run this gel? My target protein size is 2.5 kDa and 5.6 kDa.

Another question is, At the beginning I started 25 A for 45 min but after gel run some times (around 15 min) later is sowing 16 A and voltage 100. How can I maintain constant 25A? Thank you. 2020-09-24 01:10:31

Hello, thanks for your question, ok, you can heat the water and place it slowly with the urea, so it will dissolve better and will not change the volume. 2020-07-21 18:18:06