César Enrique Rivera —BIO-PROTOCOL

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Angela De Sisto Institute for Advanced Studies Foundation, IDEA, Venezuela
1 protocol

Ysvic Inojosa Institute for Advanced Studies Foundation, IDEA, Venezuela
1 protocol

Yusibeska Ramos Institute for Advanced Studies Foundation, IDEA, Venezuela
1 protocol

Laynet Puentes Energy and Enviroment, IDEA, Venezuela
1 protocol

César Enrique Rivera

Graduate, Energy and Enviroment, IDEA, Venezuela

Research focus

2 Author merit
Reviewer merit
Editor merit

Education

Bachelor, University Central of Venezuela, 2018

2 Protocols published

Isolation of PCR-quality Genomic DNA from Soils Impacted with Extra Heavy Crude Oil

Authors: Laynet A. Puentes , Yusibeska L. Ramos, Ysvic A. Inojosa, César E. Rivera and Angela De Sisto, date: 06/20/2019, view: 49, Q&A: 0

For the study of microbial communities in samples of soils impacted with extra heavy crude oil, it is necessary to perform molecular analyses. Due to the difficulty of oil matrix handling, there are very few protocols reported in writing. Also, one ...

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Very Low Molecular Weight Proteins Electrophoresis Protocol

Authors: César E. Rivera, José D. Rosales, Juan C. Freites-Perez and Eliaira Rodriguez, date: 11/20/2018, view: 1556, Q&A: 1

The electrophoresis is the most used technique to separate proteins and usually the sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) proposed by Laemmli is the prefered since it can diferentiate large size proteins, but for very ...

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Paolo Motta

University of Milan

To whom it may concern,
Good morning, I'm writing because I'm interested in trying your protocol (I need to resolve a 4 kDa protein), but I would like to ask a clarification.
In your Protein Sample Buffer recipe you add 400 mM EDTA, but EDTA solubility in water should be much lower - such a high concentration can be achieved only in very alkaline conditions (3 M NaOH according to Sigma). Is EDTA necessary for a correct run? How could I overcome this problem?
Also, I noticed that you neither add a reducing agent in your Sample Buffer nor you boil your samples, is there a particular reason for this?
Thank you very much for your availability,
Best Regards,

Paolo Motta.

2/25/2019 7:28:13 AM Reply

Good afternoon, we appreciate your interest in our protocol. In the sample buffer the EDTA was used since was the reagent available at the moment, but it can be changed for DTT at the same concentration or β-Mercaptoetanol. EDTA is not necesarry for a correct run, and we did not used reducing agent in the Sample Buffer nor boil the samples since we needed the native proteins. Best Regards.

2019-02-25 11:18:24