Home
About Us
For Authors
Submission Procedure
Preparation Guidelines
Submit Manuscript
Editorial Process
Editorial Criteria
Publishing Ethics
Competing Interests
Copyright & Permissions
Archive
My bio page
Edit user profile
Signature
Reset the password
Home
About Us
For Authors
Submission Procedure
Preparation Guidelines
Submit Manuscript
Editorial Process
Editorial Criteria
Publishing Ethics
Competing Interests
Copyright & Permissions
Archive
A peer-reviewed protocol journal. No publication fee; no access fee.
Search
Overview
Published
Edited
Reviewed
Following
Feedback
Q&A
Tetsuya Okajima
Nagoya University, Japan
4 protocols
Mitsutaka Ogawa
4 protocols
Shogo Sawaguchi
2 protocols
Ailing Zhang
1 protocol
View all coauthors >
Entzu Wan
Research focus
Developmental biology
Contributions
2
Author merit
Reviewer merit
Editor merit
2 Protocols published
A Blood-retina Barrier Permeability Assay in Young Mice Using Sulfo-NHS-LC-biotin Perfusion
Authors:
Entzu Wan
,
Mitsutaka Ogawa
and
Tetsuya Okajima
,
date:
10/20/2018,
view:
2157,
Q&A:
0
Brain and retinal vasculatures exhibit restricted vascular permeability known as blood-brain barrier and blood-retina barrier. Vascular permeability can be evaluated by perfusion of the amine reactive ester derivatives of biotin such as ...
More >
Isolation of Murine Brain and Lung Microvascular Endothelial Cells
Authors:
Ailing Zhang
,
Shogo Sawaguchi
,
Entzu Wan
,
Mitsutaka Ogawa
and
Tetsuya Okajima
,
date:
08/20/2018,
view:
2970,
Q&A:
0
This protocol describes how to isolate murine endothelial cells from newborn mice brain and 3-month-old mice lung by modifying the original protocols (Sobczak
et al.
, 2010; Ruck
et al.
, 2014). We have used the protocol to analyze ...
More >
following
More protocols >
0 Protocol feedback
Find out more
We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.