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Tetsuya Okajima
Nagoya University, Japan
4 protocols
Mitsutaka Ogawa
4 protocols
Shogo Sawaguchi
2 protocols
Ailing Zhang
1 protocol
View all coauthors >
Entzu Wan
Research focus
Developmental biology
Contributions
2
Author merit
Reviewer merit
Editor merit
2 Protocols published
A Blood-retina Barrier Permeability Assay in Young Mice Using Sulfo-NHS-LC-biotin Perfusion
Authors:
Entzu Wan
,
Mitsutaka Ogawa
and
Tetsuya Okajima
,
date:
10/20/2018,
view:
1527,
Q&A:
0
Brain and retinal vasculatures exhibit restricted vascular permeability known as blood-brain barrier and blood-retina barrier. Vascular permeability can be evaluated by perfusion of the amine reactive ester derivatives of biotin such as ...
More >
Isolation of Murine Brain and Lung Microvascular Endothelial Cells
Authors:
Ailing Zhang
,
Shogo Sawaguchi
,
Entzu Wan
,
Mitsutaka Ogawa
and
Tetsuya Okajima
,
date:
08/20/2018,
view:
2273,
Q&A:
0
This protocol describes how to isolate murine endothelial cells from newborn mice brain and 3-month-old mice lung by modifying the original protocols (Sobczak
et al.
, 2010; Ruck
et al.
, 2014). We have used the protocol to analyze ...
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