Entzu Wan —BIO-PROTOCOL

Home About Us For Authors Submit Protocol Archive

Tetsuya Okajima Nagoya University, Japan
4 protocols

Mitsutaka Ogawa 4 protocols

Shogo Sawaguchi 2 protocols

Ailing Zhang 1 protocol

Entzu Wan

2 Author merit
Reviewer merit
Editor merit

2 Protocols published

A Blood-retina Barrier Permeability Assay in Young Mice Using Sulfo-NHS-LC-biotin Perfusion

Authors: Entzu Wan, Mitsutaka Ogawa and Tetsuya Okajima, date: 10/20/2018, view: 86, Q&A: 0

Brain and retinal vasculatures exhibit restricted vascular permeability known as blood-brain barrier and blood-retina barrier. Vascular permeability can be evaluated by perfusion of the amine reactive ester derivatives of biotin such as ...

More >

Isolation of Murine Brain and Lung Microvascular Endothelial Cells

Authors: Ailing Zhang, Shogo Sawaguchi, Entzu Wan, Mitsutaka Ogawa and Tetsuya Okajima, date: 08/20/2018, view: 471, Q&A: 0

This protocol describes how to isolate murine endothelial cells from newborn mice brain and 3-month-old mice lung by modifying the original protocols (Sobczak et al., 2010; Ruck et al., 2014). We have used the protocol to analyze ...

More >