Tetsuya Okajima Nagoya University, Japan
4 protocols

Mitsutaka Ogawa 4 protocols

Shogo Sawaguchi 2 protocols

Ailing Zhang 1 protocol

Entzu Wan
Research focus
  • Developmental biology
  • 2 Author merit
2 Protocols published
A Blood-retina Barrier Permeability Assay in Young Mice Using Sulfo-NHS-LC-biotin Perfusion
Authors:  Entzu Wan, Mitsutaka Ogawa and Tetsuya Okajima, date: 10/20/2018, view: 2883, Q&A: 0
Brain and retinal vasculatures exhibit restricted vascular permeability known as blood-brain barrier and blood-retina barrier. Vascular permeability can be evaluated by perfusion of the amine reactive ester derivatives of biotin such as ...
Isolation of Murine Brain and Lung Microvascular Endothelial Cells
Authors:  Ailing Zhang, Shogo Sawaguchi, Entzu Wan, Mitsutaka Ogawa and Tetsuya Okajima, date: 08/20/2018, view: 3916, Q&A: 0
This protocol describes how to isolate murine endothelial cells from newborn mice brain and 3-month-old mice lung by modifying the original protocols (Sobczak et al., 2010; Ruck et al., 2014). We have used the protocol to analyze ...
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