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Coauthors
Douglas Gladue Agricultural Research Service Plum Island Animal Disease Center, Greenport, USA, USA,
1 protocol

Elizabeth Vuono Agricultural Research Service Plum Island Animal Disease Center, Greenport, USA, USA,
1 protocol

Elizabeth Ramirez-Medina Agricultural Research Service Plum Island Animal Disease Center, Greenport, USA, USA,
1 protocol

Keith Berggren Agricultural Research Service Plum Island Animal Disease Center, Greenport, USA, USA,
1 protocol

Manuel Victor Borca
  • Agricultural Research Service Plum Island Animal Disease Center, Greenport, USA, USA,
  • 1 Author merit

Education

DVM, Facultad de Ciencias Veterinarias, (FCV), Universidad de Buenos Aires (UBA) , Argentina, 1979

Current position

Lead Scientist, Plum Island Animal Disease Center, Agricultural Research Service, USDA, USA

Publications (since 2014)

  1. Barrionuevo, F., Di Giacomo, S., Bucafusco, D., Ayude, A., Schammas, J., Miraglia, M. C., Capozzo, A., Borca, M. V. and Perez-Filgueira, M. (2018). Systemic antibodies administered by passive immunization prevent generalization of the infection by foot-and-mouth disease virus in cattle after oronasal challenge. Virology 518: 143-151. 

  2. Borca, M. V., Holinka, L. G., Berggren, K. A. and Gladue, D. P. (2018). CRISPR-Cas9, a tool to efficiently increase the development of recombinant African swine fever viruses. Sci Rep 8(1): 3154. 

  3. Borca, M. V., O'Donnell, V., Holinka, L. G., Ramirez-Medina, E., Clark, B. A., Vuono, E. A., Berggren, K., Alfano, M., Carey, L. B., Richt, J. A., Risatti, G. R. and Gladue, D. P. (2018). The L83L ORF of African swine fever virus strain Georgia encodes for a non-essential gene that interacts with the host protein IL-1beta. Virus Res 249: 116-123. 

  4. Largo, E., Gladue, D. P., Torralba, J., Aguilella, V. M., Alcaraz, A., Borca, M. V. and Nieva, J. L. (2018). Mutation-induced changes of transmembrane pore size revealed by combined ion-channel conductance and single vesicle permeabilization analyses. Biochim Biophys Acta 1860(5): 1015-1021. 

  5. Stenfeldt, C., Arzt, J., Pacheco, J. M., Gladue, D. P., Smoliga, G. R., Silva, E. B., Rodriguez, L. L. and Borca, M. V. (2018). A partial deletion within foot-and-mouth disease virus non-structural protein 3A causes clinical attenuation in cattle but does not prevent subclinical infection. Virology 516: 115-126. 

  6. Borca, M. V., O'Donnell, V., Holinka, L. G., Sanford, B., Azzinaro, P. A., Risatti, G. R. and Gladue, D. P. (2017). Development of a fluorescent ASFV strain that retains the ability to cause disease in swine. Sci Rep 7: 46747. 

  7. Holinka, L. G., O'Donnell, V., Risatti, G. R., Azzinaro, P., Arzt, J., Stenfeldt, C., Velazquez-Salinas, L., Carlson, J., Gladue, D. P. and Borca, M. V. (2017). Early protection events in swine immunized with an experimental live attenuated classical swine fever marker vaccine, FlagT4G. PLoS One 12(5): e0177433. 

  8. O'Donnell, V., Risatti, G. R., Holinka, L. G., Krug, P. W., Carlson, J., Velazquez-Salinas, L., Azzinaro, P. A., Gladue, D. P. and Borca, M. V. (2017). Simultaneous Deletion of the 9GL and UK Genes from the African Swine Fever Virus Georgia 2007 Isolate Offers Increased Safety and Protection against Homologous Challenge. J Virol 91(1). 

  9. Borca, M. V., O'Donnell, V., Holinka, L. G., Rai, D. K., Sanford, B., Alfano, M., Carlson, J., Azzinaro, P. A., Alonso, C. and Gladue, D. P. (2016). The Ep152R ORF of African swine fever virus strain Georgia encodes for an essential gene that interacts with host protein BAG6. Virus Res 223: 181-189. 

  10. Carlson, J., O'Donnell, V., Alfano, M., Velazquez Salinas, L., Holinka, L. G., Krug, P. W., Gladue, D. P., Higgs, S. and Borca, M. V. (2016). Association of the Host Immune Response with Protection Using a Live Attenuated African Swine Fever Virus Model. Viruses 8(10). 

  11. Holinka, L. G., Largo, E., Gladue, D. P., O'Donnell, V., Risatti, G. R., Nieva, J. L. and Borca, M. V. (2016). Alteration of a Second Putative Fusion Peptide of Structural Glycoprotein E2 of Classical Swine Fever Virus Alters Virus Replication and Virulence in Swine. J Virol 90(22): 10299-10308. 

  12. O'Donnell, V., Holinka, L. G., Sanford, B., Krug, P. W., Carlson, J., Pacheco, J. M., Reese, B., Risatti, G. R., Gladue, D. P. and Borca, M. V. (2016). African swine fever virus Georgia isolate harboring deletions of 9GL and MGF360/505 genes is highly attenuated in swine but does not confer protection against parental virus challenge. Virus Res 221: 8-14. 

  13. Sanford, B., Holinka, L. G., O'Donnell, V., Krug, P. W., Carlson, J., Alfano, M., Carrillo, C., Wu, P., Lowe, A., Risatti, G. R., Gladue, D. P. and Borca, M. V. (2016). Deletion of the thymidine kinase gene induces complete attenuation of the Georgia isolate of African swine fever virus. Virus Res 213: 165-171. 

  14. Velazquez-Salinas, L., Risatti, G. R., Holinka, L. G., O'Donnell, V., Carlson, J., Alfano, M., Rodriguez, L. L., Carrillo, C., Gladue, D. P. and Borca, M. V. (2016). Recoding structural glycoprotein E2 in classical swine fever virus (CSFV) produces complete virus attenuation in swine and protects infected animals against disease. Virology 494: 178-189.

  15. Di Giacomo, S., Brito, B. P., Perez, A. M., Bucafusco, D., Pega, J., Rodriguez, L., Borca, M. V. and Perez-Filgueira, M. (2015). Heterogeneity in the Antibody Response to Foot-and-Mouth Disease Primo-vaccinated Calves. Transbound Emerg Dis 62(3): 280-287. 

  16. Fernandez-Sainz, I., Ramanathan, P., O'Donnell, V., Diaz-San Segundo, F., Velazquez-Salinas, L., Sturza, D. F., Zhu, J., de los Santos, T. and Borca, M. V. (2015). Treatment with interferon-alpha delays disease in swine infected with a highly virulent CSFV strain. Virology 483: 284-290. 

  17. Krug, P. W., Holinka, L. G., O'Donnell, V., Reese, B., Sanford, B., Fernandez-Sainz, I., Gladue, D. P., Arzt, J., Rodriguez, L., Risatti, G. R. and Borca, M. V. (2015). The progressive adaptation of a georgian isolate of African swine fever virus to vero cells leads to a gradual attenuation of virulence in swine corresponding to major modifications of the viral genome. J Virol 89(4): 2324-2332. 

  18. O'Donnell, V., Holinka, L. G., Gladue, D. P., Sanford, B., Krug, P. W., Lu, X., Arzt, J., Reese, B., Carrillo, C., Risatti, G. R. and Borca, M. V. (2015). African Swine Fever Virus Georgia Isolate Harboring Deletions of MGF360 and MGF505 Genes Is Attenuated in Swine and Confers Protection against Challenge with Virulent Parental Virus. J Virol 89(11): 6048-6056. 

  19. O'Donnell, V., Holinka, L. G., Krug, P. W., Gladue, D. P., Carlson, J., Sanford, B., Alfano, M., Kramer, E., Lu, Z., Arzt, J., Reese, B., Carrillo, C., Risatti, G. R. and Borca, M. V. (2015). African Swine Fever Virus Georgia 2007 with a Deletion of Virulence-Associated Gene 9GL (B119L), when Administered at Low Doses, Leads to Virus Attenuation in Swine and Induces an Effective Protection against Homologous Challenge. J Virol 89(16): 8556-8566. 

  20. Pega, J., Di Giacomo, S., Bucafusco, D., Schammas, J. M., Malacari, D., Barrionuevo, F., Capozzo, A. V., Rodriguez, L. L., Borca, M. V. and Perez-Filgueira, M. (2015). Systemic Foot-and-Mouth Disease Vaccination in Cattle Promotes Specific Antibody-Secreting Cells at the Respiratory Tract and Triggers Local Anamnestic Responses upon Aerosol Infection. J Virol 89(18): 9581-9590. 

  21. Fernandez-Sainz, I. J., Largo, E., Gladue, D. P., Fletcher, P., O'Donnell, V., Holinka, L. G., Carey, L. B., Lu, X., Nieva, J. L. and Borca, M. V. (2014). Effect of specific amino acid substitutions in the putative fusion peptide of structural glycoprotein E2 on Classical Swine Fever Virus replication. Virology 456-457: 121-130. 

  22. Gladue, D. P., Baker-Bransetter, R., Holinka, L. G., Fernandez-Sainz, I. J., O'Donnell, V., Fletcher, P., Lu, Z. and Borca, M. V. (2014). Interaction of CSFV E2 protein with swine host factors as detected by yeast two-hybrid system. PLoS One 9(1): e85324. 

  23. Gladue, D. P., O'Donnell, V., Baker-Bransetter, R., Pacheco, J. M., Holinka, L. G., Arzt, J., Pauszek, S., Fernandez-Sainz, I., Fletcher, P., Brocchi, E., Lu, Z., Rodriguez, L. L. and Borca, M. V. (2014). Interaction of foot-and-mouth disease virus nonstructural protein 3A with host protein DCTN3 is important for viral virulence in cattle. J Virol 88(5): 2737-2747. 

  24. Gladue, D. P., O'Donnell, V., Fernandez-Sainz, I. J., Fletcher, P., Baker-Branstetter, R., Holinka, L. G., Sanford, B., Carlson, J., Lu, Z. and Borca, M. V. (2014). Interaction of structural core protein of classical swine fever virus with endoplasmic reticulum-associated degradation pathway protein OS9. Virology 460-461: 173-179. 

  25. Holinka, L. G., Fernandez-Sainz, I., Sanford, B., O'Donnell, V., Gladue, D. P., Carlson, J., Lu, Z., Risatti, G. R. and Borca, M. V. (2014). Development of an improved live attenuated antigenic marker CSF vaccine strain candidate with an increased genetic stability. Virology 471-473: 13-18. 

  26. Largo, E., Gladue, D. P., Huarte, N., Borca, M. V. and Nieva, J. L. (2014). Pore-forming activity of pestivirus p7 in a minimal model system supports genus-specific viroporin function. Antiviral Res 101: 30-36. 

  27. O'Donnell, V., Pacheco, J. M., Larocco, M., Gladue, D. P., Pauszek, S. J., Smoliga, G., Krug, P. W., Baxt, B., Borca, M. V. and Rodriguez, L. (2014). Virus-host interactions in persistently FMDV-infected cells derived from bovine pharynx. Virology 468-470: 185-196. 

  28. Romera, S. A., Puntel, M., Quattrocchi, V., Del Medico Zajac, P., Zamorano, P., Blanco Viera, J., Carrillo, C., Chowdhury, S., Borca, M. V. and Sadir, A. M. (2014). Protection induced by a glycoprotein E-deleted bovine herpesvirus type 1 marker strain used either as an inactivated or live attenuated vaccine in cattle. BMC Vet Res 10: 8. 

  29. Stenfeldt, C., Pacheco, J. M., Borca, M. V., Rodriguez, L. L. and Arzt, J. (2014). Morphologic and phenotypic characteristics of myocarditis in two pigs infected by foot-and mouth disease virus strains of serotypes O or A. Acta Vet Scand 56: 42.

1 Protocol published
CRISPR/Cas Gene Editing of a Large DNA Virus: African Swine Fever Virus
Gene editing of large DNA viruses, such as African swine fever virus (ASFV), has traditionally relied on homologous recombination of a donor plasmid consisting of a reporter cassette with surrounding homologous viral DNA. However, this homologous ...