Emmanuel Pinteaux Division of Neuroscience and Experimental Neurology, Faculty of Biology, Medicine, and Health. University of Manchester, UK, UK,
1 protocol

Stuart Allan Division of Neuroscience and Experimental Neurology. Faculty of Biology, Medicine, and Health. University of Manchester, UK, University of Manchester, United Kingdom
2 protocols

Jonjo Miller Division of Neuroscience and Experimental Neurology, Faculty of Biology, Medicine, and Health. University of Manchester, UK, UK,
1 protocol

Catriona Cunningham Division of Neuroscience and Experimental Neurology, Faculty of Biology, Medicine, and Health. University of Manchester, UK, UK,
1 protocol

Stuart A Cain
  • Wellcome Trust Centre for Cell-Matrix Research, Division of Cell Matrix Biology & Regenerative Medicine, Faculty of Biology, Medicine, and Health, University of Manchester, UK, UK,
  • 1 Author merit

Education

Ph.D in Immunology, University of Sheffield, UK, 1997

Current position

Senior Research Associate, School of Biological Sciences, Faculty of Biology, Medicine and Health, University of Manchester, UK

Publications

  1. Massam-Wu, T., Cain, S. A. and Kielty, C. M. (2018). Extracellular Matrix Molecule-Based Capture of Mesenchymal Stromal Cells Under Flow. Methods Mol Biol 1722: 249-260.

  2. Cheng, A., Cain, S. A., Tian, P., Baldwin, A. K., Uppanan, P., Kielty, C. M. and Kimber, S. J. (2018). Recombinant Extracellular Matrix Protein Fragments Support Human Embryonic Stem Cell Chondrogenesis. Tissue Eng Part A 24(11-12): 968-978. 

  3. Leha, A., Moens, N., Meleckyte, R., Culley, O. J., Gervasio, M. K., Kerz, M., Reimer, A., Cain, S. A., Streeter, I., Folarin, A., Stegle, O., Kielty, C. M., HipSci, C., Durbin, R., Watt, F. M. and Danovi, D. (2016). A high-content platform to characterise human induced pluripotent stem cell lines. Methods 96: 85-96. 

  4. Cain, S. A., Mularczyk, E. J., Singh, M., Massam-Wu, T. and Kielty, C. M. (2016). ADAMTS-10 and -6 differentially regulate cell-cell junctions and focal adhesions. Sci Rep 6: 35956. 

  5. Holley, R. J., Tai, G., Williamson, A. J., Taylor, S., Cain, S. A., Richardson, S. M., Merry, C. L., Whetton, A. D., Kielty, C. M. and Canfield, A. E. (2015). Comparative quantification of the surfaceome of human multipotent mesenchymal progenitor cells. Stem Cell Reports 4(3): 473-488. 

  6. Banka, S., Cain, S. A., Carim, S., Daly, S. B., Urquhart, J. E., Erdem, G., Harris, J., Bottomley, M., Donnai, D., Kerr, B., Kingston, H., Superti-Furga, A., Unger, S., Ennis, H., Worthington, J., Herrick, A. L., Merry, C. L., Yue, W. W., Kielty, C. M. and Newman, W. G. (2015). Leri's pleonosteosis, a congenital rheumatic disease, results from microduplication at 8q22.1 encompassing GDF6 and SDC2 and provides insight into systemic sclerosis pathogenesis. Ann Rheum Dis 74(6): 1249-1256. 

  7. Baldwin, A. K., Cain, S. A., Lennon, R., Godwin, A., Merry, C. L. and Kielty, C. M. (2014). Epithelial-mesenchymal status influences how cells deposit fibrillin microfibrils. J Cell Sci 127(Pt 1): 158-171. 

  8. Baldwin, A. K., Simpson, A., Steer, R., Cain, S. A. and Kielty, C. M. (2013). Elastic fibres in health and disease. Expert Rev Mol Med 15: e8. 

  9. Cain, S. A., McGovern, A., Baldwin, A. K., Baldock, C. and Kielty, C. M. (2012). Fibrillin-1 mutations causing Weill-Marchesani syndrome and acromicric and geleophysic dysplasias disrupt heparan sulfate interactions. PLoS One 7(11): e48634.

  10. Marson, A., Robinson, D. E., Brookes, P. N., Mulloy, B., Wiles, M., Clark, S. J., Fielder, H. L., Collinson, L. J., Cain, S. A., Kielty, C. M., McArthur, S., Buttle, D. J., Short, R. D., Whittle, J. D. and Day, A. J. (2009). Development of a microtiter plate-based glycosaminoglycan array for the investigation of glycosaminoglycan-protein interactions. Glycobiology 19(12): 1537-1546. 

  11. Choudhury, R., McGovern, A., Ridley, C., Cain, S. A., Baldwin, A., Wang, M. C., Guo, C., Mironov, A., Jr., Drymoussi, Z., Trump, D., Shuttleworth, A., Baldock, C. and Kielty, C. M. (2009). Differential regulation of elastic fiber formation by fibulin-4 and -5. J Biol Chem 284(36): 24553-24567. 

  12. Cain, S. A., McGovern, A., Small, E., Ward, L. J., Baldock, C., Shuttleworth, A. and Kielty, C. M. (2009). Defining elastic fiber interactions by molecular fishing: an affinity purification and mass spectrometry approach. Mol Cell Proteomics 8(12): 2715-2732. 

  13. Cain, S. A., Raynal, B., Hodson, N., Shuttleworth, A. and Kielty, C. M. (2008). Biomolecular analysis of elastic fibre molecules. Methods 45(1): 42-52.

  14. Cain, S. A., Baldwin, A. K., Mahalingam, Y., Raynal, B., Jowitt, T. A., Shuttleworth, C. A., Couchman, J. R. and Kielty, C. M. (2008). Heparan sulfate regulates fibrillin-1 N- and C-terminal interactions. J Biol Chem 283(40): 27017-27027.

  15. Chaudhry, S. S., Cain, S. A., Morgan, A., Dallas, S. L., Shuttleworth, C. A. and Kielty, C. M. (2007). Fibrillin-1 regulates the bioavailability of TGFbeta1. J Cell Biol 176(3): 355-367. 

  16. Bax, D. V., Mahalingam, Y., Cain, S., Mellody, K., Freeman, L., Younger, K., Shuttleworth, C. A., Humphries, M. J., Couchman, J. R. and Kielty, C. M. (2007). Cell adhesion to fibrillin-1: identification of an Arg-Gly-Asp-dependent synergy region and a heparin-binding site that regulates focal adhesion formation. J Cell Sci 120(Pt 8): 1383-1392.

  17. Cain, S. A., Morgan, A., Sherratt, M. J., Ball, S. G., Shuttleworth, C. A. and Kielty, C. M. (2006). Proteomic analysis of fibrillin-rich microfibrils. Proteomics 6(1): 111-122. 

  18. Mellody, K. T., Freeman, L. J., Baldock, C., Jowitt, T. A., Siegler, V., Raynal, B. D., Cain, S. A., Wess, T. J., Shuttleworth, C. A. and Kielty, C. M. (2006). Marfan syndrome-causing mutations in fibrillin-1 result in gross morphological alterations and highlight the structural importance of the second hybrid domain. J Biol Chem 281(42): 31854-31862. 

  19. Baldock, C., Siegler, V., Bax, D. V., Cain, S. A., Mellody, K. T., Marson, A., Haston, J. L., Berry, R., Wang, M. C., Grossmann, J. G., Roessle, M., Kielty, C. M. and Wess, T. J. (2006). Nanostructure of fibrillin-1 reveals compact conformation of EGF arrays and mechanism for extensibility. Proc Natl Acad Sci U S A 103(32): 11922-11927. 

  20. Marson, A., Rock, M. J., Cain, S. A., Freeman, L. J., Morgan, A., Mellody, K., Shuttleworth, C. A., Baldock, C. and Kielty, C. M. (2005). Homotypic fibrillin-1 interactions in microfibril assembly. J Biol Chem 280(6): 5013-5021. 

  21. Higginbottom, A., Cain, S. A., Woodruff, T. M., Proctor, L. M., Madala, P. K., Tyndall, J. D., Taylor, S. M., Fairlie, D. P. and Monk, P. N. (2005). Comparative agonist/antagonist responses in mutant human C5a receptors define the ligand binding site. J Biol Chem 280(18): 17831-17840. 

  22. Clarke, A. W., Wise, S. G., Cain, S. A., Kielty, C. M. and Weiss, A. S. (2005). Coacervation is promoted by molecular interactions between the PF2 segment of fibrillin-1 and the domain 4 region of tropoelastin. Biochemistry 44(30): 10271-10281. 

  23. Cain, S. A., Baldock, C., Gallagher, J., Morgan, A., Bax, D. V., Weiss, A. S., Shuttleworth, C. A. and Kielty, C. M. (2005). Fibrillin-1 interactions with heparin. Implications for microfibril and elastic fiber assembly. J Biol Chem 280(34): 30526-30537. 

  24. Rock, M. J., Cain, S. A., Freeman, L. J., Morgan, A., Mellody, K., Marson, A., Shuttleworth, C. A., Weiss, A. S. and Kielty, C. M. (2004). Molecular basis of elastic fiber formation. Critical interactions and a tropoelastin-fibrillin-1 cross-link. J Biol Chem 279(22): 23748-23758. 

  25. Rennen, H. J., Oyen, W. J., Cain, S. A., Monk, P. N., Corstens, F. H. and Boerman, O. C. (2003). Tc-99m-labeled C5a and C5a des Arg74 for infection imaging. Nucl Med Biol 30(3): 267-272. 

  26. Kalant, D., Cain, S. A., Maslowska, M., Sniderman, A. D., Cianflone, K. and Monk, P. N. (2003). The chemoattractant receptor-like protein C5L2 binds the C3a des-Arg77/acylation-stimulating protein. J Biol Chem 278(13): 11123-11129.

  27. Francis, K., Lewis, B. M., Akatsu, H., Monk, P. N., Cain, S. A., Scanlon, M. F., Morgan, B. P., Ham, J. and Gasque, P. (2003). Complement C3a receptors in the pituitary gland: a novel pathway by which an innate immune molecule releases hormones involved in the control of inflammation. FASEB J 17(15): 2266-2268. 

  28. Cain, S. A., Higginbottom, A. and Monk, P. N. (2003). Characterisation of C5a receptor agonists from phage display libraries. Biochem Pharmacol 66(9): 1833-1840. 

  29. Cain, S. A. and Monk, P. N. (2002). The orphan receptor C5L2 has high affinity binding sites for complement fragments C5a and C5a des-Arg(74). J Biol Chem 277(9): 7165-7169. 

  30. Cain, S. A., Woodruff, T. M., Taylor, S. M., Fairlie, D. P., Sanderson, S. D. and Monk, P. N. (2001). Modulation of ligand selectivity by mutation of the first extracellular loop of the human C5a receptor. Biochem Pharmacol 61(12): 1571-1579. 

  31. Cain, S. A., Williams, D. M., Harris, V. and Monk, P. N. (2001). Selection of novel ligands from a whole-molecule randomly mutated C5a library. Protein Eng 14(3): 189-193. 

  32. Cain, S. A., Coughlan, T. and Monk, P. N. (2001). Mapping the ligand-binding site on the C5a receptor: arginine74 of C5a contacts aspartate282 of the C5a receptor. Biochemistry 40(46): 14047-14052. 

  33. Cain, S. A., Ratcliffe, C. F., Williams, D. M., Harris, V. and Monk, P. N. (2000). Analysis of receptor/ligand interactions using whole-molecule randomly-mutated ligand libraries. J Immunol Methods 245(1-2): 139-145. 

  34. Crass, T., Bautsch, W., Cain, S. A., Pease, J. E. and Monk, P. N. (1999). Receptor activation by human C5a des Arg74 but not intact C5a is dependent on an interaction between Glu199 of the receptor and Lys68 of the ligand. Biochemistry 38(30): 9712-9717. 

  35. Paczkowski, N. J., Finch, A. M., Whitmore, J. B., Short, A. J., Wong, A. K., Monk, P. N., Cain, S. A., Fairlie, D. P. and Taylor, S. M. (1999). Pharmacological characterization of antagonists of the C5a receptor. Br J Pharmacol 128(7): 1461-1466. 

  36. Sayers, I., Cain, S. A., Swan, J. R., Pickett, M. A., Watt, P. J., Holgate, S. T., Padlan, E. A., Schuck, P. and Helm, B. A. (1998). Amino acid residues that influence Fc epsilon RI-mediated effector functions of human immunoglobulin E. Biochemistry 37(46): 16152-16164. 

  37. Helm, B. A., Sayers, I., Swan, J., Smyth, L. J., Cain, S. A., Suter, M., Machado, D. C., Spivey, A. C. and Padlan, E. A. (1998). Protein and cell engineering of components of the human immunoglobulin E receptor/effector system: applications for therapy and diagnosis. Technol Health Care 6(2-3): 195-207.

1 Protocol published
Generation of Human Mesenchymal Stem Cell 3D Spheroids Using Low-binding Plates
The 3D culture of human mesenchymal stem cells (hMSCs) represents a more physiological environment than classical 2D culture and has been used to enhance the MSC secretome or extend cell survival after transplantation. Here we describe a simple and ...
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