Yasuomi Tada Division of Biological Science, Graduate School of Science, Nagoya University, Japan, Japan,
1 protocol

Mika Nomoto Division of Biological Science, Graduate School of Science, Nagoya university, Japan, Japan,
1 protocol

Hironaka Tsukagoshi
  • Faculty, Faculty of Agriculture, Meijo University, Japan, Japan,
Research focus
  • Molecular biology
  • 1 Author merit


Ph.D. in Agricultural Science, Graduate School of Bio-Agricultural Sciences, Nagoya University, Japan

Current position

Associate professor, Faculty of Agriculture, Meijo University, Japan


  1. Sakaoka, S., Mabuchi, K., Morikami, A. and Tsukagoshi, H. (2018).  MYB30 regulates root cell elongation under abscisic acid signaling. Commun Integr Biol 11(4):e1526604. 
  2. Mabuchi, K., Maki, H., Itaya, T., Suzuki, T., Nomoto, M., Sakaoka, S., Morikami, A., Higashiyama, T., Tada, Y., Busch, W. and Tsukagoshi, H. (2018). MYB30 links ROS signaling, root cell elongation, and plant immune responses. Proc Natl Acad Sci U S A 115(20):E4710-E4719. 
  3. Nishijima, T., Furuhashi, M., Sakaoka, S., Morikami, A. and Tsukagoshi, H. (2017). Ectopic expression of Mesembryanthemum crystallinum sodium transporter McHKT2 provides salt stress tolerance in Arabidopsis thaliana. Biosci Biotechnol Biochem 81(11):2139-2144. 
  4. Tsukagoshi, H. (2016). Control of root growth and development by reactive oxygen species. Curr Opin Plant Biol 29:57-63. 
  5. Tsukagoshi, H.*, Suzuki, T., Nishikawa, K., Agarie, S., Ishiguro, S. and Higashiyama, T. (2015). RNA-seq analysis of the response of the halophyte, Mesembryanthemum crystallinum (ice plant) to high salinity. PLoS One 10(2):e0118339. (*Corresponding author)
  6. Ruduś, I., Terai, H., Shimizu, T., Kojima, H., Hattori, K., Nishimori, Y., Tsukagoshi, H., Kamiya, Y., Seo, M., Nakamura, K., Kępczyński, J. and Ishiguro, S. (2014). Wound-induced expression of DEFECTIVE IN ANTHER DEHISCENCE1 and DAD1-like lipase genes is mediated by both CORONATINE INSENSITIVE1-dependent and independent pathways in Arabidopsis thaliana. Plant Cell Rep 33(6):849-860.
  7. Tsukagoshi H. (2012). Defective root growth triggered by oxidative stress is controlled through the expression of cell cycle-related genes. Plant Sci 197:30-39. 
  8. Iyer-Pascuzzi, A.S., Jackson, T., Cui, H., Petricka, J.J., Busch, W., Tsukagoshi, H. and Benfey, P.N. (2011). Cell identity regulators link development and stress responses in the Arabidopsis root. Dev Cell 21(4):770-782. 
  9. Tsukagoshi, H., Busch, W. and Benfey, P.N. (2010). Transcriptional regulation of ROS controls transition from proliferation to differentiation in the root. Cell 143(4):606-616. 
  10. Long, T.A., Tsukagoshi, H., Busch, W., Lahner, B., Salt, D.E. and Benfey, P.N. (2010). The bHLH transcription factor POPEYE regulates response to iron deficiency in Arabidopsis roots. Plant Cell 22(7):2219-2236.
  11. Maeo, K., Tokuda, T., Ayame, A., Mitsui, N., Kawai, T., Tsukagoshi, H., Ishiguro, S. and Nakamura, K. (2009). An AP2-type transcription factor, WRINKLED1, of Arabidopsis thaliana binds to the AW-box sequence conserved among proximal upstream regions of genes involved in fatty acid synthesis. Plant J 60(3):476-487. 
  12. Terakura, S., Ueno, Y., Tagami, H., Kitakura, S., Machida, C., Wabiko, H., Aiba, H., Otten, L., Tsukagoshi, H., Nakamura, K. and Machida, Y. (2007). An oncoprotein from the plant pathogen agrobacterium has histone chaperone-like activity. Plant Cell 19(9):2855-2865.
  13. Tsukagoshi, H., Morikami, A. and Nakamura, K. (2007). Two B3 domain transcriptional repressors prevent sugar-inducible expression of seed maturation genes in Arabidopsis seedlings. Proc Natl Acad Sci U S A 104(7):2543-2547. 
  14. Masaki, T., Tsukagoshi, H., Mitsui, N., Nishii, T., Hattori, T., Morikami, A. and Nakamura, K. (2005). Activation tagging of a gene for a protein with novel class of CCT-domain activates expression of a subset of sugar-inducible genes in Arabidopsis thaliana. Plant J 43(1):142-152.
  15. Shimizu, M., Igasaki, T., Yamada, M., Yuasa, K., Hasegawa, J., Kato, T., Tsukagoshi, H., Nakamura, K., Fukuda, H. and Matsuoka, K. (2005). Experimental determination of proline hydroxylation and hydroxyproline arabinogalactosylation motifs in secretory proteins. Plant J 42(6):877-889.
  16. Tsukagoshi, H., Saijo, T., Shibata, D., Morikami, A. and Nakamura, K. (2005). Analysis of a sugar response mutant of Arabidopsis identified a novel B3 domain protein that functions as an active transcriptional repressor. Plant Physiol 138(2):675-685. 
  17. Masaki, T., Mitsui, N., Tsukagoshi, H., Nishii, T., Morikami, A. and Nakamura, K. (2005). ACTIVATOR of Spomin::LUC1/WRINKLED1 of Arabidopsis thaliana transactivates sugar-inducible promoters. Plant Cell Physiol 46(4):547-556.
1 Protocol published
In vitro Protein-DNA Binding Assay (AlphaScreen® Technology)
Authors:  Mika Nomoto, Yasuomi Tada and Hironaka Tsukagoshi, date: 02/05/2019, view: 5127, Q&A: 0
Identification of specific DNA binding sites of transcription factors is important in understanding their functions. Recent techniques allow us to investigate genome-wide in vivo binding positions by chromatin immunoprecipitation combined ...
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