Lukas Synek —BIO-PROTOCOL

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Heribert Hirt Desert Agriculture Initiative, King Abdullah University of Science and Technology, Kingdom of Saudi Arabia, Kingdom of Saudi Arabia,
1 protocol

Eleonora Rolli Institut of Plant Sciences Paris-Saclay (IPS2), , UMR 9213/UMR1403, CNRS, INRA, Université Paris-Sud, Université d'Evry, Université Paris-Diderot, Sorbonne Paris-Cité, , France, France,
1 protocol

Axel de Zelicourt Institut of Plant Sciences Paris-Saclay (IPS2), , UMR 9213/UMR1403, CNRS, INRA, Université Paris-Sud, Université d'Evry, Université Paris-Diderot, Sorbonne Paris-Cité, , France, France,
1 protocol

Maged Saad Desert Agriculture Initiative, King Abdullah University of Science and Technology, Kingdom of Saudi Arabia, Kingdom of Saudi Arabia,
1 protocol

Lukas Synek

Desert Agriculture Initiative, King Abdullah University of Science and Technology, Kingdom of Saudi Arabia, Kingdom of Saudi Arabia,

Research focus

Microbiology

1 Author merit
Reviewer merit
Editor merit

1 Protocol published

Quantification of Root Colonizing Bacteria

Authors: Maged M. Saad, Axel de Zélicourt, Eleonora Rolli , Lukas Synek and Heribert Hirt , date: 07/20/2018, view: 3565, Q&A: 1

Here we describe a simple method to quantify the number of viable bacteria (e.g., Enterobacter sp. SA187) that colonize the root system of Arabidopsis thaliana.

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Muahammad Ahmad

Hi,
Very helpful protocol
Just a short question: Is the 1/2 MS medium with sugar or without sugar? Thank you

3/16/2021 6:42:49 AM Reply

Muahammad Ahmad

Thank you for the response.

Best Regards
Ahmad

2021-03-31 06:58:47

Axel de Zelicourt

Institut of Plant Sciences Paris-Saclay (IPS2), , UMR 9213/UMR1403, CNRS, INRA, Université Paris-Sud, Université d'Evry, Université Paris-Diderot, Sorbonne Paris-Cité, , France, France,

hello Muhammad

we used the MS medium basal salt and nothing else (no addition of vitamins or hormones) and of absolutely no sugar at all, as the bacteria would develop extremely rapidly and cover your plant :)

2021-03-17 01:12:27