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Heribert Hirt
Desert Agriculture Initiative, King Abdullah University of Science and Technology, Kingdom of Saudi Arabia, Kingdom of Saudi Arabia,
1 protocol
Lukas Synek
Desert Agriculture Initiative, King Abdullah University of Science and Technology, Kingdom of Saudi Arabia, Kingdom of Saudi Arabia,
1 protocol
Eleonora Rolli
Institut of Plant Sciences Paris-Saclay (IPS2), , UMR 9213/UMR1403, CNRS, INRA, Université Paris-Sud, Université d'Evry, Université Paris-Diderot, Sorbonne Paris-Cité, , France, France,
1 protocol
Maged Saad
Desert Agriculture Initiative, King Abdullah University of Science and Technology, Kingdom of Saudi Arabia, Kingdom of Saudi Arabia,
1 protocol
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Axel de Zelicourt
Institut of Plant Sciences Paris-Saclay (IPS2), , UMR 9213/UMR1403, CNRS, INRA, Université Paris-Sud, Université d'Evry, Université Paris-Diderot, Sorbonne Paris-Cité, , France, France,
Research focus
Microbiology
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1
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1 Protocol published
Quantification of Root Colonizing Bacteria
Authors:
Maged M. Saad
,
Axel de Zélicourt
,
Eleonora Rolli
,
Lukas Synek
and
Heribert Hirt
,
date:
07/20/2018,
view:
3480,
Q&A:
1
Here we describe a simple method to quantify the number of viable bacteria (
e.g.
,
Enterobacter
sp. SA187) that colonize the root system of
Arabidopsis thaliana
.
More >
Muahammad Ahmad
Hi,
Very helpful protocol
Just a short question: Is the 1/2 MS medium with sugar or without sugar? Thank you
3/16/2021 6:42:49 AM
Reply
Muahammad Ahmad
Thank you for the response.
Best Regards
Ahmad
2021-03-31 06:58:47
Axel de Zelicourt
Institut of Plant Sciences Paris-Saclay (IPS2), , UMR 9213/UMR1403, CNRS, INRA, Université Paris-Sud, Université d'Evry, Université Paris-Diderot, Sorbonne Paris-Cité, , France, France,
hello Muhammad
we used the MS medium basal salt and nothing else (no addition of vitamins or hormones) and of absolutely no sugar at all, as the bacteria would develop extremely rapidly and cover your plant :)
2021-03-17 01:12:27
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