Camilo Berríos Pastén
  • UChile
Research focus
  • Microbiology
1 Protocol feedback
Colony Immunoblotting Assay for Detection of Bacterial Cell-surface or Extracellular Proteins
Authors:  Timo A. Lehti and Benita Westerlund-Wikström, date: 09/05/2013, view: 17847, Q&A: 1
This simple protocol describes how to detect antigens from agar-grown bacterial colonies transferred to nitrocellulose using specific antibodies. The protocol is well suitable for detection of bacterial proteins exposed on the cell surface or secreted to the extracellular space and it can be modified also for detection of intracellular proteins. ...
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