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A peer-reviewed protocol journal. No publication fee; no access fee.
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Chiara Ambrogio
University of Turin
31 protocols
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Reviewer
Muhammad Aslam
Faculty, Justus Liebig University, Giessen Germany
Research focus
Cell biology
GTPases, endothelial barrier function
Contributions
Reviewer merit
Editor merit
Education
Habilitation, Justus Liebig University, 2016
Lab information
Muhammad Aslam's Lab
2 Protocols reviewed
Measuring Small-molecule Inhibition of Protein Interactions in Live Cells Using FLIM-FRET
Authors:
James M. Pemberton
,
Qian Liu
and
David W. Andrews
,
date:
10/20/2019,
view:
3107,
Q&A:
0
This protocol was designed to quantitatively measure small-molecule displacement of proteins in live mammalian cells using fluorescence lifetime imaging microscopy–Förster resonance energy transfer (FLIM-FRET). Tumour cell survival is often ...
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Determination of Cellular Uptake and Endocytic Pathways
Authors:
Jing Gong
,
Hong-Xia Wang
and
Kam W. Leong
,
date:
02/20/2019,
view:
4839,
Q&A:
0
Efficiency of drug and gene delivery via nonviral vehicles is contingent on proper cellular uptake and intracellular release. Further, various cargos, such as nucleases for gene editing or inhibitors for endosomal receptors, require transport to ...
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