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Alexandros Katranidis Forschungszentrum Jülich, Institute of Complex Systems ICS-5, Germany, Germany,
1 protocol

Michele Cerminara Institute of Complex Systems ICS-5, Forschungszentrum Jülich, Germany, Germany,
1 protocol

Mayuri Sadoine Forschungszentrum Jülich, Institute of Complex Systems ICS-5, Germany, Germany,
1 protocol

Jörg Fitter
  • Forschungszentrum Jülich, Institute of Complex Systems ICS-5, Germany, Germany,
  • 1 Author merit

Education

Dr. rer. nat. in Physics, Free University of Berlin, Germany

Current position

Professor in Biophysics, I. Physikalisches Institut (IA), Biophysik, RWTH Aachen University, Germany

Publications (since 2010)

  1. Sadoine, M., Cerminara, M., Gerrits, M., Fitter, J. and Katranidis, A. (2018). Cotranslational Incorporation into Proteins of a Fluorophore Suitable for smFRET Studies. ACS Synth Biol 7(2): 405-411.
  2. Sadoine, M., Cerminara, M., Kempf, N., Gerrits, M., Fitter, J. and Katranidis, A. (2017). Selective Double-Labeling of Cell-Free Synthesized Proteins for More Accurate smFRET Studies. Anal Chem 89(21): 11278-11285.
  3. Kempf, N., Remes, C., Ledesch, R., Zuchner, T., Hofig, H., Ritter, I., Katranidis, A. and Fitter, J. (2017). A Novel Method to Evaluate Ribosomal Performance in Cell-Free Protein Synthesis Systems. Sci Rep 7: 46753.
  4. Kempe, D., Cerminara, M., Poblete, S., Schone, A., Gabba, M. and Fitter, J. (2017). Single-Molecule FRET Measurements in Additive-Enriched Aqueous Solutions. Anal Chem 89(1): 694-702.
  5. Baumann, A., Kerruth, S., Fitter, J., Buldt, G., Heberle, J., Schlesinger, R. and Ataka, K. (2016). In-Situ Observation of Membrane Protein Folding during Cell-Free Expression. PLoS One 11(3): e0151051.
  6. Kempe, D., Schone, A., Fitter, J. and Gabba, M. (2015). Accurate fluorescence quantum yield determination by fluorescence correlation spectroscopy. J Phys Chem B 119(13): 4668-4672.
  7. Stadler, A. M., Koza, M. M. and Fitter, J. (2015). Determination of conformational entropy of fully and partially folded conformations of holo- and apomyoglobin. J Phys Chem B 119(1): 72-82.
  8. Hofig, H., Gabba, M., Poblete, S., Kempe, D. and Fitter, J. (2014). Inter-dye distance distributions studied by a combination of single-molecule FRET-filtered lifetime measurements and a weighted accessible volume (wAV) algorithm. Molecules 19(12): 19269-19291.
  9. Gabba, M., Poblete, S., Rosenkranz, T., Katranidis, A., Kempe, D., Zuchner, T., Winkler, R. G., Gompper, G. and Fitter, J. (2014). Conformational state distributions and catalytically relevant dynamics of a hinge-bending enzyme studied by single-molecule FRET and a coarse-grained simulation. Biophys J 107(8): 1913-1923.
  10. Lamprou, P., Kempe, D., Katranidis, A., Buldt, G. and Fitter, J. (2014). Nanosecond dynamics of calmodulin and ribosome-bound nascent chains studied by time-resolved fluorescence anisotropy. Chembiochem 15(7): 977-985.
  11. Stadler, A. M., Pellegrini, E., Johnson, M., Fitter, J. and Zaccai, G. (2012). Dynamics-stability relationships in apo- and holomyoglobin: a combined neutron scattering and molecular dynamics simulations study. Biophys J 102(2): 351-359.
  12. Rosenkranz, T., Schlesinger, R., Gabba, M. and Fitter, J. (2011). Native and unfolded states of phosphoglycerate kinase studied by single-molecule FRET. Chemphyschem 12(3): 704-710.
  13. Kumari, A., Rosenkranz, T., Fitter, J. and Kayastha, A. M. (2011). Structural stability of soybean (Glycine max) alpha-amylase: properties of the unfolding transition studied with fluorescence and CD spectroscopy. Protein Pept Lett 18(3): 253-260.
  14. Fitter, J., Katranidis,  A., Rosenkranz, T., Atta, D., Schlesinger, R. and Büldt,  G. (2011) Single molecule fluorescence spectroscopy: A tool for protein studies approaching cellular environmental conditions. Soft Matter 7, 1254-1259.
  15. Kulishova, L., Dimoula, K., Jordan, M., Wirtz, A., Hofmann, D., Santigo-Schübel, B., Fitter, J., Pohl,M. and Spiess, A.C. (2010) Factors influencing the operational stability of NADPH-dependent alcohol dehydrogenase and an NADH-dependent variant thereof in gas/solid reactors. J. Mol. Cat. B 67, 271-283
  16. Kumari, A., Singh, V. K., Fitter, J., Polen, T. and Kayastha, A. M. (2010). Alpha-amylase from germinating soybean (Glycine max) seeds--purification, characterization and sequential similarity of conserved and catalytic amino acid residues. Phytochemistry 71(14-15): 1657-1666.
  17. Kumari, A., Rosenkranz, T., Kayastha, A. M. and Fitter, J. (2010). The effect of calcium binding on the unfolding barrier: A kinetic study on homologous alpha-amylases. Biophys Chem 151(1-2): 54-60.
  18. Inoue, R., Biehl, R., Rosenkranz, T., Fitter, J., Monkenbusch, M., Radulescu, A., Farago, B. and Richter, D. (2010). Large domain fluctuations on 50-ns timescale enable catalytic activity in phosphoglycerate kinase. Biophys J 99(7): 2309-2317.
1 Protocol published
Preparation of Cell-free Synthesized Proteins Selectively Double Labeled for Single-molecule FRET Studies
Authors:  Mayuri Sadoine, Michele Cerminara, Jörg Fitter and Alexandros Katranidis, date: 06/20/2018, view: 1074, Q&A: 0
Single-molecule FRET (smFRET) is a powerful tool to investigate molecular structures and conformational changes of biological molecules. The technique requires protein samples that are site-specifically equipped with a pair of donor and acceptor ...