Arthur Zanetti+Nunes+Fernandes
  • USP
Research focus
  • Biochemistry
1 Protocol feedback
The Inoue Method for Preparation and Transformation of Competent E. coli: "Ultra Competent" Cells
Author:  Hogune Im, date: 10/20/2011, view: 22978, Q&A: 1
This protocol differs from other procedures in that the bacterial culture is grown at 18 °C rather than the conventional 37 °C. Otherwise, the protocol is unremarkable and follows a fairly standard course. Why growing the cells at low temperature should affect the efficiency of transformation is unknown. Perhaps the composition or the physical ...
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