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Coauthors
Ping Zheng 1 protocol

Yu Wang Key Laboratory of Systems Microbial Biotechnology, Chinese Academy of Sciences, People’s Republic of China, People’s Republic of China,
1 protocol

Jiao Liu Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences
1 protocol

Jibin Sun
  • Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences
  • 1 Author merit

Education

PhD, German Research Center for Biotechnology (GBF), Germany, 2004

Current position

Professor, Systems & Synthetic Biotechnology Laboratory, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, China

Publications

  1. Zheng, X., Zheng, P., Zhang, K., Cairns, T. C., Meyer, V., Sun, J. and Ma, Y. (2018). 5S rRNA Promoter for Guide RNA Expression Enabled Highly Efficient CRISPR/Cas9 Genome Editing in Aspergillus niger. ACS Synth Biol

  2. Zheng, X., Zheng, P., Sun, J., Kun, Z. and Ma, Y. (2018). Heterologous and endogenous U6 snRNA promoters enable CRISPR/Cas9 mediated genome editing in Aspergillus niger. Fungal Biol Biotechnol 5: 2. 

  3. Zhang, Q., Zheng, X., Wang, Y., Yu, J., Zhang, Z., Dele-Osibanjo, T., Zheng, P., Sun, J., Jia, S. and Ma, Y. (2018). Comprehensive optimization of the metabolomic methodology for metabolite profiling of Corynebacterium glutamicum. Appl Microbiol Biotechnol. 

  4. Wang, Y., Liu, Y., Liu, J., Guo, Y., Fan, L., Ni, X., Zheng, X., Wang, M., Zheng, P., Sun, J. and Ma, Y. (2018). MACBETH: Multiplex automated Corynebacterium glutamicum base editing method. Metab Eng 47: 200-210. 

  5. Wang, Y., Cao, G., Xu, D., Fan, L., Wu, X., Ni, X., Zhao, S., Zheng, P., Sun, J. and Ma, Y. (2018). A Novel Corynebacterium glutamicum l-Glutamate Exporter. Appl Environ Microbiol 84(6). 

  6. Wang, X. C., Liu, J., Zhao, J., Ni, X. M., Zheng, P., Guo, X., Sun, C. M., Sun, J. B. and Ma, Y. H. (2018). Efficient production of trans-4-hydroxy-l-proline from glucose using a new trans-proline 4-hydroxylase in Escherichia coli. J Biosci Bioeng

  7. Tuyishime, P., Wang, Y., Fan, L., Zhang, Q., Li, Q., Zheng, P., Sun, J. and Ma, Y. (2018). Engineering Corynebacterium glutamicum for methanol-dependent growth and glutamate production. Metab Eng 49: 220-231. 

  8. Wang, X., Wang, Y., Liu, J., Li, Q., Zhang, Z., Zheng, P., Lu, F. and Sun, J. (2017). Biological conversion of methanol by evolved Escherichia coli carrying a linear methanol assimilation pathway. Bioresources and Bioprocessing 4(1): 41. 

  9. Liu, J., Wang, Y., Lu, Y., Zheng, P., Sun, J. and Ma, Y. (2017). Development of a CRISPR/Cas9 genome editing toolbox for Corynebacterium glutamicum. Microb Cell Fact 16(1): 205. 

  10. Wang, Y., Li, Q., Zheng, P., Guo, Y., Wang, L., Zhang, T., Sun, J. and Ma, Y. (2016). Evolving the L-lysine high-producing strain of Escherichia coli using a newly developed high-throughput screening method. J Ind Microbiol Biotechnol 43(9): 1227-1235. 

  11. Kou, F., Zhao, J., Liu, J., Shen, J., Ye, Q., Zheng, P., Li, Z., Sun, J. and Ma, Y. (2016). Characterization of a new lysine decarboxylase from Aliivibrio salmonicida for cadaverine production at alkaline pH. Journal of Molecular Catalysis B: Enzymatic 133: S88-S94. 

  12. Wu, Y., Li, P., Zheng, P., Zhou, W., Chen, N. and Sun, J. (2015). Complete genome sequence of Corynebacterium glutamicum B253, a Chinese lysine-producing strain. J Biotechnol 207: 10-11. 

  13. Liu, Y., Li, Q., Zheng, P., Zhang, Z., Liu, Y., Sun, C., Cao, G., Zhou, W., Wang, X., Zhang, D., Zhang, T., Sun, J. and Ma, Y. (2015). Developing a high-throughput screening method for threonine overproduction based on an artificial promoter. Microb Cell Fact 14: 121. 

  14. Xu, Z., Zheng, P., Sun, J. and Ma, Y. (2013). ReacKnock: identifying reaction deletion strategies for microbial strain optimization based on genome-scale metabolic network. PLoS One 8(12): e72150. 

  15. Geng, F., Chen, Z., Zheng, P., Sun, J. and Zeng, A. P. (2013). Exploring the allosteric mechanism of dihydrodipicolinate synthase by reverse engineering of the allosteric inhibitor binding sites and its application for lysine production. Appl Microbiol Biotechnol 97(5): 1963-1971. 

  16. Chen, L., Chen, Z., Zheng, P., Sun, J. and Zeng, A. P. (2013). Study and reengineering of the binding sites and allosteric regulation of biosynthetic threonine deaminase by isoleucine and valine in Escherichia coli. Appl Microbiol Biotechnol 97(7): 2939-2949. 

1 Protocol published
CRISPR/Cas9-mediated ssDNA Recombineering in Corynebacterium glutamicum
Authors:  Jiao Liu, Yu Wang, Ping Zheng and Jibin Sun, date: 10/05/2018, view: 73, Q&A: 0
Corynebacterium glutamicum is a versatile workhorse for industrial bioproduction of many kinds of chemicals and fuels, notably amino acids. Development of advanced genetic engineering tools is urgently demanded for systems metabolic ...