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Chaoxi Luo
1 protocol
Tao Chen
1 protocol
Yufu Wang
1 protocol
Weixiao Yin
Huazhong Agricultural University
1 protocol
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Yang Lin
Research focus
Plant science
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1
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1 Protocol published
Functional Evaluation of the Signal Peptides of Secreted Proteins
Authors:
Weixiao Yin
,
Yufu Wang
,
Tao Chen
,
Yang Lin
and
Chaoxi Luo
,
date:
05/05/2018,
view:
5267,
Q&A:
2
Here, we describe a method that can be used to evaluate the function of predicted signal peptides. This method utilizes the yeast
Saccharomyces cerevisiae
YTK12 strain and pSUC2 vector in which the pSUC2 vector with fused predicted signal ...
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Rajdeep Jaswal
Rajdeep Jaswal
I have tried with 2% raffinose, all the clones were growing easily. Should I reduce the concentration of raffinose in YPRAA medium?
At 2% I am not getting any positive selection. mentioned here.
3/23/2019 7:36:05 AM
Reply
Kakashi Hatake
JAAS
Actually, antimycin A is indispensible in YPRAA, if you omit antimycin from YPRAA, even YTK12 and YTK12 transformed with empty vector can grow well.
2019-12-13 09:53:15
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