Gerald Schwank Gerald Schwank
1 protocol

Jonathan Hall 1 protocol

Katja Bargsten 1 protocol

Christian Berk 1 protocol

Martin Jinek
  • 2 Author merit
2 Protocols published
In vitro Generation of CRISPR-Cas9 Complexes with Covalently Bound Repair Templates for Genome Editing in Mammalian Cells
The CRISPR-Cas9 system is a powerful genome-editing tool that promises application for gene editing therapies. The Cas9 nuclease is directed to the DNA by a programmable single guide (sg)RNA, and introduces a site-specific double-stranded break ...
Heterologous Expression and Purification of the CRISPR-Cas12a/Cpf1 Protein
Authors:  Prarthana Mohanraju, John van der Oost, Martin Jinek and Daan C. Swarts, date: 05/05/2018, view: 2494, Q&A: 3
This protocol provides step by step instructions (Figure 1) for heterologous expression of Francisella novicida Cas12a (previously known as Cpf1) in Escherichia coli. It additionally includes a protocol for high-purity purification ...
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