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浩 都
16 protocols
立仲 熊
作物遗传改良国家重点实验室, 华中农业大学, 武汉, 武汉,
22 protocols
玉洁 方
5 protocols
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游均
Research focus
Plant science
Contributions
3
Author merit
Reviewer merit
Editor merit
3 Protocols published
活性氧检测
Authors:
游均
,
方玉洁
and
熊立仲
,
date:
04/27/2018,
view:
6931,
Q&A:
0
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圈圈 史
浙江师范大学
1/5/2020 3:09:03 PM
Reply
圈圈 史
浙江师范大学
你好,最近才看到消息,回的有些晚了。我过几天打算做下水稻根的DAB染色,关于方法,我是这样做将植株浸于含有0.2%DAB的10毫摩尔MESbuffer中(ph为6.5),抽真空十分钟后,样品避光静置8小时,,最后用95%的酒精退染,并保存在新的酒精中。不知道您的实验做成功了吗?方法是否和我的一样,我的材料也是水稻根尖
2020-08-06 19:50:10
若辰 聂
海南大学
您好,冒昧的问下您根尖染色的问题解决了吗?我看其它的水浴是为了脱叶绿素,所以根应该不用脱色吧,因为我最近打算做水稻幼苗根的染色实验(不切段,整个幼苗培养染根),所以如果您也做了类似实验的话,想向您询问下相关步骤,感激不尽!
2020-07-08 16:24:51
水稻离体叶片失水速率测定
Authors:
都浩
,
游均
and
熊立仲
,
date:
04/27/2018,
view:
2734,
Q&A:
0
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