Tagnon Missihoun
  • Faculty, Université du Québec à Trois-Rivières
Research focus
  • Plant science
2 Protocols feedback
Histochemical Detection of Superoxide and H2O2 Accumulation in Brassica juncea Seedlings
Authors:  Deepak Kumar, Mohd Aslam Yusuf, Preeti Singh, Meryam Sardar and Neera Bhalla Sarin, date: 04/20/2014, view: 14206, Q&A: 0
Plant cells continually produce reactive oxygen species (ROS) as a by-product of aerobic metabolism. Increased production of ROS occurs under unfavorable conditions imposed by various abiotic and biotic factors. Accumulation of ROS is damaging to various cellular components and macromolecules including plasma membrane, nucleic acids, and proteins ...
Perls Staining for Histochemical Detection of Iron in Plant Samples
Authors:  Tzvetina Brumbarova and Rumen Ivanov, date: 09/20/2014, view: 10663, Q&A: 2
Visualization of iron (Fe) localization in plants has greatly enhanced our understanding of plant Fe homeostasis. One of the relatively simple and yet powerful techniques is the classical Perls blue stain (Perls, 1867). The technique is based on the conversion of ferrocyanide to insoluble crystals of Prussian blue in the presence of Fe3+ ...
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