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Coauthors
Cláudia Bispo Parnassus Flow Cytometry Core (PFCC), University of California, USA, USA,
1 protocol

Cassandra Adams Genome Analysis Core (GAC), University of California, USA, USA,
1 protocol

Oyang Teng Genome Analysis Core (GAC), University of California, United States, United States,
1 protocol

Kirsten Copren Genome Analysis Core (GAC), University of California, USA, USA,
1 protocol

Michael Lee
  • Parnassus Flow Cytometry Core (PFCC), University of California, USA, USA,
  • 1 Author merit
1 Protocol published
Quantifying Gene Expression Directly from FACS Using Hydrolysis (TaqMan) Probes on Flash-frozen Cells
Authors:  Kirsten A. Copren, Oyang Teng, Cassandra J. Adams, Cláudia Bispo and Michael Lee, date: 04/20/2018, view: 208, Q&A: 0
The TaqMan Gene Expression Cells-to-CtTM Kit enables reverse transcription directly from cell lysates without the need for isolating RNA. The recommended input range for the lysis reaction is between 10-105 cells, though the ...