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Cláudia Bispo Parnassus Flow Cytometry Core (PFCC), University of California, USA, USA,
1 protocol

Cassandra Adams Genome Analysis Core (GAC), University of California, USA, USA,
1 protocol

Oyang Teng Genome Analysis Core (GAC), University of California, United States, United States,
1 protocol

Kirsten Copren Genome Analysis Core (GAC), University of California, USA, USA,
1 protocol

Michael Lee

Parnassus Flow Cytometry Core (PFCC), University of California, USA, USA,

Research focus

Molecular biology

1 Author merit
Reviewer merit
Editor merit

1 Protocol published

Quantifying Gene Expression Directly from FACS Using Hydrolysis (TaqMan) Probes on Flash-frozen Cells

Authors: Kirsten A. Copren, Oyang Teng, Cassandra J. Adams, Cláudia Bispo and Michael Lee, date: 04/20/2018, view: 5479, Q&A: 0

The TaqMan Gene Expression Cells-to-Ct^TM Kit enables reverse transcription directly from cell lysates without the need for isolating RNA. The recommended input range for the lysis reaction is between 10-10⁵ cells, though the ...

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