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Cláudia Bispo
Parnassus Flow Cytometry Core (PFCC), University of California, USA, USA,
1 protocol
Cassandra Adams
Genome Analysis Core (GAC), University of California, USA, USA,
1 protocol
Oyang Teng
Genome Analysis Core (GAC), University of California, United States, United States,
1 protocol
Kirsten Copren
Genome Analysis Core (GAC), University of California, USA, USA,
1 protocol
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Michael Lee
Parnassus Flow Cytometry Core (PFCC), University of California, USA, USA,
Research focus
Molecular biology
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1
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1 Protocol published
Quantifying Gene Expression Directly from FACS Using Hydrolysis (TaqMan) Probes on Flash-frozen Cells
Authors:
Kirsten A. Copren
,
Oyang Teng
,
Cassandra J. Adams
,
Cláudia Bispo
and
Michael Lee
,
date:
04/20/2018,
view:
5479,
Q&A:
0
The TaqMan Gene Expression Cells-to-Ct
TM
Kit enables reverse transcription directly from cell lysates without the need for isolating RNA. The recommended input range for the lysis reaction is between 10-10
5
cells, though the ...
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