Seijiro Ono Experimental Farm, National Institute of Genetics, Mishima, Japan, Japan,
1 protocol

Ken-Ichi Nonomura
  • Experimental Farm, National Institute of Genetics, Mishima, Japan, Japan,
  • 1 Author merit


PhD in Agriculture, Graduate school of genetic resources technology, Japan, 1996

Current position

Associate Professor, Experimental Farm, National Institute of Genetics, Japan


  1. Ono, S., Liu, H., Tsuda, K., Fukai, E., Tanaka, K., Sasaki, T. and Nonomura, K. I. (2018). EAT1 transcription factor, a non-cell-autonomous regulator of pollen production, activates meiotic small RNA biogenesis in rice anther tapetum. PLoS Genet 14(2): e1007238.
  2. Tsuda, K., Abraham-Juarez, M. J., Maeno, A., Dong, Z., Aromdee, D., Meeley, R., Shiroishi, T., Nonomura, K. I. and Hake, S. (2017). KNOTTED1 cofactors, BLH12 and BLH14, regulate internode patterning and vein anastomosis in maize. Plant Cell 29(5): 1105-1118.
  3. Liu, H. and Nonomura, K. I. (2016). A wide reprogramming of histone H3 modifications during male meiosis I in rice is dependent on the Argonaute protein MEL1. J Cell Sci 129(19): 3553-3561.
  4. Orn, C., Shishido, R., Akimoto, M., Ishikawa, R., Htun, T. M., Nonomura, K., Koide, Y., Sarom, M., Vang, S., Sophany, S., Makara, O. and Ishii, T. (2015). Evaluation of genetic variation among wild rice populations in Cambodia. Breed Sci 65(5): 430-437.
  5. Niihama, M., Mochizuki, M., Kurata, N. and Nonomura, K. (2015). PCR-based INDEL markers co-dominant between Oryza sativa, japonica cultivars and closely-related wild Oryza species. Breed Sci 65(4): 357-361.
  6. Miyazaki, S., Sato, Y., Asano, T., Nagamura, Y. and Nonomura, K. (2015). Rice MEL2, the RNA recognition motif (RRM) protein, binds in vitro to meiosis-expressed genes containing U-rich RNA consensus sequences in the 3'-UTR. Plant Mol Biol 89(3): 293-307.
  7. Komiya, R., Ohyanagi, H., Niihama, M., Watanabe, T., Nakano, M., Kurata, N. and Nonomura, K. (2014). Rice germline-specific Argonaute MEL1 protein binds to phasiRNAs generated from more than 700 lincRNAs. Plant J 78(3): 385-397.
  8. Yamaki, S., Ohyanagi, H., Yamasaki, M., Eiguchi, M., Miyabayashi, T., Kubo, T., Kurata, N. and Nonomura, K. (2013). Development of INDEL markers to discriminate all genome types rapidly in the genus Oryza. Breed Sci 63(3): 246-254.
  9. Ueda, K., Yoshimura, F., Miyao, A., Hirochika, H., Nonomura, K. and Wabiko, H. (2013). Collapsed abnormal pollen1 gene encoding the Arabinokinase-like protein is involved in pollen development in rice. Plant Physiol 162(2): 858-871.
  10. Yamaki, S., Nagato, Y., Kurata, N. and Nonomura, K. (2011). Ovule is a lateral organ finally differentiated from the terminating floral meristem in rice. Dev Biol 351(1): 208-216.
  11. Nonomura, K., Eiguchi, M., Nakano, M., Takashima, K., Komeda, N., Fukuchi, S., Miyazaki, S., Miyao, A., Hirochika, H. and Kurata, N. (2011). A novel RNA-recognition-motif protein is required for premeiotic G1/S-phase transition in rice (Oryza sativa L.). PLoS Genet 7(1): e1001265.
  12. Yamazaki, Y., Sakaniwa, S., Tsuchiya, R., Nonomura, K.-I. and Kurata, N. (2010). Oryzabase: an integrated information resource for rice science. Breeding Science 60(5): 544-548.
  13. Yamaki, S., Miyabayashi, T., Eiguchi, M., Kitano, H., Nonomura, K.-I. and Kurata, N. (2010). Diversity of panicle branching patterns in wild relatives of rice. Breeding Science 60(5): 586-596.
  14. Nonomura, K. I., Morishima, H., Miyabayashi, T., Yamaki, S., Eiguchi, M., Kubo, T. Kurata, N. (2010). The wild Oryza collection in National BioResource Project (NBRP) of Japan: History, biodiversity and utility. Breed. Sci 60(5): 502-508.
  15. Nonomura, K., Morohoshi, A., Nakano, M., Eiguchi, M., Miyao, A., Hirochika, H. and Kurata, N. (2007). A germ cell specific gene of the ARGONAUTE family is essential for the progression of premeiotic mitosis and meiosis during sporogenesis in rice. Plant Cell 19(8): 2583-2594.
  16. Miyabayashi, T., Nonomura, K.-I., Morishima, H. and Kurata, N. (2007). Genome size of twenty wild species of Oryza determined by flow cytometric and chromosome analyses. Breeding Science 57(1): 73-78.
  17. Nonomura, K., Nakano, M., Eiguchi, M., Suzuki, T. and Kurata, N. (2006). PAIR2 is essential for homologous chromosome synapsis in rice meiosis I. J Cell Sci 119(Pt 2): 217-225.
  18. Nonomura, K. I., Nakano, M., Murata, K., Miyoshi, K., Eiguchi, M., Miyao, A., Hirochika, H. and Kurata, N. (2004). An insertional mutation in the rice PAIR2 gene, the ortholog of Arabidopsis ASY1, results in a defect in homologous chromosome pairing during meiosis. Mol Genet Genomics 271(2): 121-129.
  19. Nonomura, K., Nakano, M., Fukuda, T., Eiguchi, M., Miyao, A., Hirochika, H. and Kurata, N. (2004). The novel gene HOMOLOGOUS PAIRING ABERRATION IN RICE MEIOSIS1 of rice encodes a putative coiled-coil protein required for homologous chromosome pairing in meiosis. Plant Cell 16(4): 1008-1020.
  20. Nonomura, K., Miyoshi, K., Eiguchi, M., Suzuki, T., Miyao, A., Hirochika, H. and Kurata, N. (2003). The MSP1 gene is necessary to restrict the number of cells entering into male and female sporogenesis and to initiate anther wall formation in rice. Plant Cell 15(8): 1728-1739.
  21. Nonomura, K. and Kurata, N. (2001). The centromere composition of multiple repetitive sequences on rice chromosome 5. Chromosoma 110(4): 284-291.
  22. Nonomura, K. I. and Kurata, N. (1999). Organization of the 1.9-kb repeat unit RCE1 in the centromeric region of rice chromosomes. Mol Gen Genet 261(1): 1-10.
  23. Yasui, H., Nonomura, K.I. Iwata, N. (1997). Detection of alien Oryza punctata Kotschy chromosomes in rice, Oryza sativa L., by genomic in situ hybridization. J. Fac. Agr 42(1-2): 63-68.
  24. Nonomura, K.-I., Yoshimura, A. and Iwata, N. (1997). Cytogenetical gene mapping by reciprocal translocation and tertiary trisomic analyses in rice (Oryza sativa L.). Genes & Genetic Systems 72(1): 41-49.
  25. Nonomura, K.-I., Yoshimura, A., Kawasaki, T. and Iwata, N. (1994). Production of tertiary trisomics in rice (Oryza sative L.).Japanese Journal of Breeding 44(2): 137-142.
1 Protocol published
Microscopic Observation of Subcellular GFP-tagged Protein Localization in Rice Anthers
Authors:  Seijiro Ono and Ken-Ichi Nonomura, date: 08/05/2018, view: 56, Q&A: 0
This protocol demonstrates a simple method to determine the subcellular localization of fluorescence-tagged proteins on the vibratome sections of rice developing anthers. If a cell type-specific promoter is used to drive the tagged protein-encoding ...