Vincent Rotello University of Massachusetts, USA
2 protocols
Alessandro Didonna University of California, San Francisco
27 protocols

Julie Weidner University of Göteborg
3 protocols

Reviewer
Rubul Mout
  • Post-Doc, Institute for Protein Design, University of Washington
Research focus
  • Biochemistry
  • Protein design, protein engineering, gene editing, nanotechnology
  • 2 Author merit

Education

PhD, University of Massachusetts, 2017

Lab information

David Baker lab
https://www.bakerlab.org/

Publications

2 Protocols published
A General Method for Intracellular Protein Delivery through ‘E-tag’ Protein Engineering and Arginine Functionalized Gold Nanoparticles
Authors:  Rubul Mout and Vincent M. Rotello, date: 12/20/2017, view: 3744, Q&A: 0
In this protocol, we describe a method for direct cytosolic protein delivery that avoids endosomal entrapment of the delivered proteins. We achieved this by tagging the desired protein with an oligo glutamic acid tag (E-tag), and subsequently using ...
Cytosolic and Nuclear Delivery of CRISPR/Cas9-ribonucleoprotein for Gene Editing Using Arginine Functionalized Gold Nanoparticles
Authors:  Rubul Mout and Vincent M. Rotello, date: 10/20/2017, view: 6447, Q&A: 1
In this protocol, engineered Cas9-ribonucleoprotein (Cas9 protein and sgRNA, together called Cas9-RNP) and gold nanoparticles are used to make nanoassemblies that are employed to deliver Cas9-RNP into cell cytoplasm and nucleus. Cas9 protein is ...
1 Protocol reviewed
Wheat Germ Agglutinin (WGA)-SDS-PAGE: A Novel Method for the Detection of O-GlcNAc-modified Proteins by Lectin Affinity Gel Electrophoresis
Authors:  Ko Fujioka, Yuji Kubota and Mutsuhiro Takekawa, date: 12/05/2018, view: 2063, Q&A: 0
Diverse cytoplasmic and nuclear proteins dynamically change their molecular functions by O-linked β-N-acetylglucosamine (O-GlcNAc) modification on serine and/or threonine residues. Evaluation of the O-GlcNAcylation level of a specific protein, ...
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