Hiba Zahreddine,+Ph.D.
  • University of Montreal
Research focus
  • Cancer biology
1 Protocol feedback
Antibody Purification from Western Blotting
Author:  Lin Fang, date: 03/20/2012, view: 13487, Q&A: 2
This protocol describes a method of purifying antibodies from sera with denatured antigens immobilized on western blot membranes. Advantages include (1) fast and easy; (2) purification of antibody with antigen in denatured form allows high yield in case antigen protein solubility is limited. Disadvantage is that possible antibodies that recognize ...
We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.