Hiba Zahreddine,+Ph.D.
  • University of Montreal
Research focus
  • Cancer biology
1 Protocol feedback
Antibody Purification from Western Blotting
Author:  Lin Fang, date: 03/20/2012, view: 13014, Q&A: 2
This protocol describes a method of purifying antibodies from sera with denatured antigens immobilized on western blot membranes. Advantages include (1) fast and easy; (2) purification of antibody with antigen in denatured form allows high yield in case antigen protein solubility is limited. Disadvantage is that possible antibodies that recognize ...