Reviewer
Eduardo Rosa-Molinar
  • Faculty, The University of Kansas
Research focus
  • Neuroscience
  • Rosa-Molinar and his group focuses on developing/refining reagents, technologies, and workflows based on analytical electron microcopy, specifically, scanning transmission electron microscopy and electron spectroscopy, in order to determine the three-dimensional nano-scale geometry and chemical composition of synapses. We are particularly interested in “mixed synapses”, a poorly studied synapse that combines the features of both chemical and electrical synapses (i.e., gap junction).

Education

Ph.D., The University of Nebraska Medical Center, 1997

Lab information

Rosa-Molinar Lab
https://pharmtox.ku.edu/rosa-molinar-lab

Publications

PUBLICATIONS (names that are underlined indicate current and former laboratory members, including technicians, undergraduate and graduate students, and post-doctoral fellows)
Peer-Reviewed Papers and Extended Abstracts (submitted/in revision)
1. Shinogle-Decker, H., Martinez-Rivera, N., O’Brien, J., Powell, R. D., Joshi, V. N., Connell, S., and Rosa- Molinar, E.. Correlative FRET: New Method Improves Rigor and Reproducibility in Determining Distances within Synaptic Architecture. J. Biomed. Optics (submitted)
2. P. S. Thapa-Chetri, C. M. Santiago-Robles, N. Martinez-Rivera, I. I. Torres-Vazquez, V. N. Joshi, R. D. Powell, and E. Rosa-Molinar. Development and Characterization of Nanoparticles as Imaging Probes for Correlative Optical and Electron Microscopy. IEEE TNANO (submitted)
3. Martínez-Rivera, N., J. L. Serrano-Vélez, I. I. Torres-Vázquez, and E. Rosa-Molinar. Revealing a spinal microcircuit for sensorimotor integration of an unpaired sexually dimorphic fin movement during a fast innate behavior: Are superficial neuromasts proprioceptors? Biotechnic and Histochemistry (submitted).
4. Jose L. Serrano-Velez, Melanie Rodriguez-Alvarado, Noraida Martinez-Rivera, Irma I. Torres- Vazquez, and Eduardo Rosa-Molinar. Dissection and analytics of a neural circuit: From neurons- to-dendrites-to-connexin35-containing gap junction puncta-to-connectivity. Biotechnic and Histochemistry (submitted)
5. Serrano-Velez, J. L., N. Martinez-Rivera, M. Rodriguez-Alvarado, C. Santiago-Robles, I. I. Torres- Vazquez, N. L. Rivera-Rivera, S. E. Fraser, and E. Rosa-Molinar. Dye-coupling revisited. Biotechnic and Histochemistry (submitted)

Peer-Reviewed Extended Abstracts in Peer-Reviewed Journals and Trade Magazines in Optics, Photonics, and Microscopy (published)
1. Clymer, M. and E. Rosa-Molinar (2014). Microscopy Shows Its True Colors. Biophotonics
21(8): 28-30
2. Franke, T., J.L. Serrano-Velez, N.L. Rivera-Rivera, E. Rosa-Molinar, and M. Langhorst (2014). Simplified Approach Makes Two-Photon Multicolor Imaging Less Costly. Biophotonics 21(2):20-23 (Cover photograph for issue).
3. Rosa-Molinar, E. (2012). Mixed Synapses as a Testbed for Developing Reagents for Building Connectomes and Synaptomes. Microsc. Microanal. 2012 Aug; 18 (S02):624-625; doi 10.1017/S1431927612004977.
4. Torres-Vazquez, I. I., Serrano-Velez, J. L., Rosa-Molinar, E., Orange, F., Guinel, M. J., Koutis, I., Wolf, J., Laimer, C., Joshi, V., and Powell, R. D. (2012). EnzMet™: An Enzymatic Metallography Reagent for Accurately Delineating Neuronal Boundaries for Segmenting Gap Junction-Coupled Neurons in their Three-dimensional Space. Microsc. Microanal. 2012 Aug; 18 (S02):660-661; doi 10.1017/S1431927612005156.
5. Liu, W., Mitra, D., Joshi, V., Powell, R.D., Hainfeld, J., Serrano-Velez, J. L., Torres-Vasquez, I. I., Rosa-Molinar, E., and Takvorian, P. (2011). EnzMet for Versatile, Highly Sensitive Light and Electron Microscopy Staining. Microsc. Microanal. 2011 Aug; 17(S02):116-117; doi 10.1017/S1431927611001450.
6. Serrano-Velez, J. L., Mancuso, J., Torres-Vazquez, I. I., and Rosa-Molinar, E. (2009). Serial Block- face Scanning Electron Microscopic Correlative Methods for Mapping Neuronal Interconnectivity. Microsc. Microanal. 2009 Aug; 15(S02):624-625; doi 10.1017/S1431927609099036.
7. Mancuso, J., Serrano-Velez, J. L., Torres-Vazquez, I. I., and Rosa-Molinar, E. (2009). Three- Dimensional Correlated Microscopy of Fluorescence Microscopy and Serial Block-Face SEM. Microsc. Microanal. 2009 Aug;15 (S02):38-39; doi 10.1017/S1431927609099024.
8. Vallejo, P., Rodriguez, A., Fonseca, L. F., Rosa-Molinar, E., and Posada, Y. (2005). Nanocrystalline Silicon Clusters for the Imaging of Dynamic Cellular Processes. Microsc. Microanal. 2005 Aug; 11(S02):1122-1123; doi 10.1017/S1431927605510018.
9. Serrano-Velez, J. L. and Rosa-Molinar, E. (2005). Dendritic Fields of Ventrolateral Spinal Motor Column Motor Neurons Show Scaled Growth During Anterior Transposition of the Western Mosquitofish, Gambusia affinis affinis Sexually Dimorphic Anal Fin: A Study of Morphometric Properties. Microsc. Microanal. 2005 Aug;11(S02):1120-1121; doi 10.1017/S1431927605509930.
10. Rivera, J. M., Betancourt, J. E., Rosario, M.G., and Rosa-Molinar, E. (2005). Synthesis, Characterization and Bioimaging Applications of Multi-functional Dendrimer-based Probes. Microsc. Microanal. 2005 Aug;11(S02):1112-1113; doi 10.1017/S1431927605508079.
11. Rosa-Molinar E. and Serrano-Velez, J. L. (2005). Novel Multi-Functional Correlative Neural Tract Tracers for Elucidating the Developing Nervous System in Thick Biological Specimens Using WideField Fluorescence 3-D Deconvolution Microscopy. Microsc. Microanal. 2005 Aug;11(S02):11081109; doi 10.1017/S1431927605509887.
12. Rodríguez-Cortes, A., Dugger, B. N., Rivera-Rivera, N. L., Serrano-Velez, J. L., and Rosa-Molinar, E. (2005). Improving the DiOlistics Imaging Technique: Visualizing Ventral Horn Spinal Motor Neurons in the Western Mosquitofish, Gambusia affinis affinis. Microsc. Microanal. 2005 Aug;11(S02):11041105; doi:10.1017/S1431927605509929; doi 10.1017/S1431927604887385.
13. Rosa-Molinar, E., Rodriguez-Cortes, A., Serrano Velez, J. L., Rosario-Garcia, M. G., and Rivera- Ortiz, J. M. (2004). Advances in Preparative and Labeling Technology for Microscopy and Imaging of the Developing Nervous System: Transport Studies of Novel Multi-Functional Correlative Probes. Microsc. Microanal. 2004 Aug; 10(S02):1214-1215; doi 10.1017/S1431927604887385.

Peer-Reviewed Papers (published)
1. Hewitt, S. M., Baskin, D. G., Frevert, C. W., Stahl, W. L., and Rosa-Molinar, E. (2014). Controls for immunohistochemistry: Controls for Immunohistochemistry: The Histochemical Society's Standards of Practice for Validation of Immunohistochemical Assays. J. Histochem. Cytochem. Jul 14. pii: 0022155414545224. [Epub ahead of print] PMID: 25023613.
2. Serrano-Velez, J. L., Rodriguez-Alvarado M., Torres-Vazquez I. I., Fraser, S. E., Yasumura, T., Vanderpool, K. G., Rash, J. E. and Rosa-Molinar, E. (2014). Abundance of gap junctions at glutamatergic mixed synapses in adult Mosquitofish spinal cord neurons. Front. Neural Circuits 8 (66):1-16; doi: 10.3389/fncir.2014.0006.
3. Rivera-Rivera, N. L., Martinez-Rivera, N., Torres-Vázquez, I. I., Serrano-Vélez, J. L., Lauder, G. V., and Rosa-Molinar, E. (2010). Remodeling of a Male Poecillid Fish Body Plan Results in Sexually Dimorphic Behavior and Nervous System: The Rapid Copulatory Circuit. Int. Comp. Biol. 50(6), pp. 1081–1090; doi 10.1093/Icb/Icq147.
4. Betancourt, J. E., C. Subramani, J. L. Serrano-Velez, E. Rosa-Molinar, V. M. Rotello, and J. M. Rivera (2010). Drug Encapsulation within Self-assembled Microglobules Formed by Thermoresponsive Supramolecules. Chem. Commun. 46, 8537-8539; doi 10.1039/c0cc04063k.
5. Rosario-Ortiz, L, S. Rivera-Pabon, I. Torres-Vázquez, L. Diaz-Perez, L. Pesquera-Diaz, N. L. RiveraRivera, E. Colon-Garcia, J. L. Serrano-Vélez, and E. Rosa-Molinar (2008). The Western Mosquitofish, Gambusia affinis affinis: A New Laboratory Animal Resource for the Study of Sexual Dimorphism in Neural Circuits. Lab Animal 37(6), 1-7; doi 10.1038/laban0608-263.
6. Rosa-Molinar, E., Krumlauf, R. K., and M. B. Pritz (2005). Hindbrain Development and Evolution: Past Present and Future. Brain Behav. Evol. 66(4):219-221; doi 10.1159/000088126.
7. Fritzsch, B., Gregory, D., and Rosa-Molinar, E. (2005). The Development of Hindbrain Afferent Projections: Evidence of Timed and Specific Ingrowth of Fibers. Zoology 108(4):297-306; doi 10.1016/j.zool.2005.08.003.
8. McCaffery, P.J., J. Adams, M. Maden, and E. Rosa-Molinar (2003). Too Much of a Good Thing: Retinoic Acid as an Endogenous Regulator of Neural Differentiation and Exogenous Teratogen. Eur. J. Neurosci. 18(3):457-472; doi 10.1046/j.1460-9568.2003.02765.x.
9. Burke, A. C. and E. Rosa-Molinar. (2002). Starting from fins: Parallelism in the evolution of limbs and genitalia; the fin-to-limb transition. Evo. Dev. 4 (5):375-377; doi 10.1046/j.1525- 142X.2002.02024.x.
10. Rosa-Molinar, E. and A. C. Burke (2002). Starting from fins: Parallelism in the evolution of limbs and genitalia; the fin-to-genitalia transition. Evo. Devo. 4(2) 1-3; doi 10.1046/j.1525- 142X.2002.01067.x.
11. Rosa-Molinar, E. B. J. Proskocil, M. Ettel, and B. Fritzsch (1999). Whole-mount Procedures for Simultaneous Visualization of Nerves, Neurons, Cartilage, and Bone. Brain Res. Brain Res. Protoc. 4:115-123; doi 10.1016/S1385-299x(99)00007-0. (Cover photograph for journal volume).
12. Rosa-Molinar, E., B. J. Proskocil, S. E. Hendricks, and B. Fritzsch (1998). A Mechanism for Anterior Transposition of the Anal Fin and its Appendicular Support in the Western Mosquitofish, Gambusia affinis affinis (Baird and Girard, 1854). Acta Anat. 163:75-91; PMID 9873137.
13. Rosa-Molinar, E., P. McCaffery, and B. Fritzsch (1997). Sex Differences in Endogenous Retinoid Release in the Post-Embryonic Spinal Cord of the Western Mosquitofish, Gambusia affinis affinis. Adv. Exp. Med. Biol. 414:95-108; PMID 9059611.
14. Rosa-Molinar, E., B. Fritzsch, and S. E. Hendricks (1996). The Organizational-Activational Concept Revisited: Sexual Differentiation in an Atherinomorph Teleost. Horm. Behav. 30:563-575; doi 10.1006/hbeh.1996.0059.
15. Rosa-Molinar, E., S. E. Hendricks, J. F. Rodriguez-Sierra, and B. Fritzsch (1994). The Development of the Anal fin Appendicular Support in the Western Mosquitofish, Gambusia affinis affinis (Baird and Girard, 1854): A Reinvestigation and Reinterpretation. Acta Anat. 151:20-35; PMID 7879590. (Cover photograph for journal volume).
16. Rosa-Molinar, E. and C. S. Williams (1984). Notes on Fecundity of an Arrhenoid Population of Mosquitofish, Gambusia affinis holbrookii (Rosen and Bailey, 1963). Northeast Gulf Sci. 7 (1): 121- 125.
17. Rosa-Molinar, E., G. Howard and C. S. Williams (1984). Notes on the use of Y-Modulation Imaging in Studies of Cuticular Surfaces of Larval (Philometridae) Nematodes. J. Electr. Microsc. Tech. 1:311-312; doi 10.1002/jemt.1060010310.
18. Rosa-Molinar, E., C. S. Williams and J. R. Lichtenfels (1983). Larval Nematodes (Philometridae) Granuloma In the ovaries of the Black-tip Shark, Carcharhinus limbatus (Valenciennes). J. Wildli. Dis. 19 (3):275-277; PMID 6644926.
19. Rosa-Molinar, E., C. S. Williams and S. Collard (1983). Fetal Mummification in the Sandbar Shark,
Carcharhinus plumbeus (Nardo 1827). J. Wildli Dis. 19 (2): 156-158; PMID 6684186.
20. Rosa-Molinar, E. (1982). A Two-headed shark fetus, Carcharhinus plumbeus (Muller and Henle): A Case Report. J. Alabama Acad. Sci. 53:17-20.
21. Rosa-Molinar, E. and S. B. Ruth (1981). First Record of the Pink Wormfish, Microdesmus longipinnis
in Alabama Waters. J. Alabama Acad. Sci. 52:212.

Peer-Reviewed Book Chapter[s] (published)
1. Martinez-Rivera, N., Serrano-Velez, J. L., Lauder, G. V. and Rosa-Molinar E. (2010). New Insights into Gambusia Behavior. For: Viviparous Fishes III, M. Uribe and H.J. Grier, editors, New Life Publications, Inc., Homestead, FL, pp. 13-30
2. Rosa-Molinar, E. (2005). Edwin S. Goodrich's Theory of Transposition Revisited: The Shift to a Sexually Dimorphic Axial Formulae and Nervous System in a Poeciliine Fish. For: Viviparous Fishes II, M. Uribe and H.J. Grier, editors, New Life Publications, Inc., Homestead, FL, pp. 60-70
3. Rodriguez-Sierra, J. F. and E. Rosa-Molinar (1990). Sexual Dimorphism in the Organization of Central and Peripheral Mechanisms Controlling Sexual Behavior in the Mosquitofish, Gambusia affinis affinis. In: Balthazart, J. (ed.), Comp. Physiol., Vol. 8., Hormones, Brain, and Behavior in Vertebrates. 1. Sexual Differentiation, Neuroanatomical Aspects, Neurotransmitters And Neuropeptides; International Conference On Hormones, Brain and Behaviour, Liege, Belgium, August 18-22,. Ix 226p. S. Karger Ag: Basel, Switzerland; New York, New York, USA. Illus. 64-81.
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