Nhien Tran
  • PhD student
Research focus
  • Molecular biology
2 Protocols feedback
Generation of microRNA Sponge Library
Author:  Sebastian Herzog, date: 04/20/2018, view: 1230, Q&A: 0
This protocol describes the generation and functional validation of microRNA (miRNA) sponge or decoy constructs. When expressed from a strong promoter, these transcripts can sequester specific miRNA:RISC complexes, thereby resulting in a derepression of endogenous target mRNA. Hence, cells expressing such sponges display a partial or full miRNA ...
Rapid Screening and Evaluation of Maize Seedling Resistance to Stalk Rot Caused by Fusarium spp.
Authors:  Yali Sun, Xinsen Ruan, Liang Ma, Fang Wang and Xiquan Gao, date: 05/20/2018, view: 1346, Q&A: 0
Corn stalk rot caused by Fusarium spp., a genus of soil-borne fungal pathogens, has become a major concern of maize production. This disease normally causes significant reduction of maize yield and quality worldwide. The field assay for identifying stalk rot resistance using adult plants is largely relying on large population, yet ...
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