Stéphanie Jaubert-Possamai

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Bruno Favery ISA, INRA, Université Côte d’Azur, CNRS, France
2 protocols

Michaël Quentin ISA, INRA, Université Côte d’Azur, CNRS, France
2 protocols

Marie-Noëlle Rosso 1 protocol

Chinh-Nghia Nguyen 1 protocol

Stéphanie Jaubert-Possamai

1 Author merit
Reviewer merit
Editor merit

1 Protocol published

In situ Hybridization (ISH) in Preparasitic and Parasitic Stages of the Plant-parasitic Nematode Meloidogyne spp.

Authors: Maëlle Jaouannet, Chinh-Nghia Nguyen, Michaël Quentin, Stéphanie Jaubert-Possamai, Marie-Noëlle Rosso and Bruno Favery, date: 03/20/2018, view: 3473, Q&A: 1

The spatio-temporal expression pattern of a gene provides important indications to better understand its biological function. In situ hybridization (ISH) uses a labeled complementary single-stranded RNA or DNA probe to localize gene ...

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Lei Zhang

Washington State University

Formaldehyde for fixing nematodes
Since the formaldehyde recommended in the protocol has been discontinued by Sigma, I tried a similar product from sigma: 37% formaldehyde solution (Sigma: 252549), and it worked well for the in situ hybridization.

12/18/2018 12:15:00 PM Reply

Lei Zhang

Washington State University

Dear Dr. Favery,

Thanks very much for your help and suggestion on the in situ hybridization!

Lei

2018-12-19 09:00:56

Bruno Favery

ISA, INRA, Université Côte d’Azur, CNRS, France

Thank you for sharing this information.

2018-12-19 02:27:55