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Aashiq Kachroo
Concordia University
1 protocol
Andrew Ellington
1 protocol
Riddhiman Garge
1 protocol
Elizabeth Gardner
1 protocol
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Edward M Marcotte
Research focus
Microbiology
Contributions
1
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1 Protocol published
Single-step Precision Genome Editing in Yeast Using CRISPR-Cas9
Authors:
Azat Akhmetov
,
Jon M Laurent
,
Jimmy Gollihar
,
Elizabeth C Gardner
,
Riddhiman K Garge
,
Andrew D Ellington
,
Aashiq H Kachroo
and
Edward M Marcotte
,
date:
03/20/2018,
view:
7994,
Q&A:
1
Genome modification in budding yeast has been extremely successful largely due to its highly efficient homology-directed DNA repair machinery. Several methods for modifying the yeast genome have previously been described, many of them involving at ...
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Aaron Rosenbloom
Yale University
I tried to do the second Golden Gate Reaction in silico before doing the experiment, and it did not work. Is it possible that the vector needed is pYTK095 instead of pYTK083?
12/23/2019 8:46:31 AM
Reply
Aashiq Kachroo
Concordia University
Hi Aaron
Thanks for pointing the error. You are right, the second round Golden Gate cloning uses pYTK095 plasmid. We will correct the error soon.
2019-12-24 06:14:58
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