Dear Prashanth
Thank you for your response. Please check the Composition of Vogel Bonner Medium. The right composition is:
1. MgSo4.7h20-10.0gm
2. Citric Acid Monohydrate-100gm
3 K2HPo4-500gm
4- NaHNH4Po4.4H20-175gm
Distilled Water to make vol. one litre.
The Composition of Agar used for solidification is 1-2%, Generally 1.5%, So the Amount would be 15 Gram for 1 litre and 7.5 gm for half litre. Please check your composition.
BTW, Please let me Know this is review paper?
Please make the necessary changes.
7/13/2018 10:00:08 PM Reply