Waldemar Vollmer Institute for Cell and Molecular Biosciences, Newcastle University, NE2 4AX, Newcastle upon Tyne
2 protocols

Katharina Peters 1 protocol

Christian Otten 1 protocol

Manuel Pazos
  • 2 Author merit
2 Protocols published
Optimized Protocol for the Incorporation of FDAA (HADA Labeling) for in situ Labeling of Peptidoglycan
Authors:  Katharina Peters, Manuel Pazos, Michael S. VanNieuwenhze and Waldemar Vollmer, date: 08/05/2019, view: 778, Q&A: 0
The essential peptidoglycan (PG) layer surrounds the cytoplasmic membrane in nearly all bacteria. It is needed to maintain the shape of the cell and protect it from lysis due to high turgor. Growth of the PG layer is a complex process that involves ...
Bacterial Cell Wall Precursor Phosphatase Assays Using Thin-layer Chromatography (TLC) and High Pressure Liquid Chromatography (HPLC)
Authors:  Manuel Pazos, Christian Otten and Waldemar Vollmer, date: 03/20/2018, view: 2591, Q&A: 0
Peptidoglycan encases the bacterial cytoplasmic membrane to protect the cell from lysis due to the turgor. The final steps of peptidoglycan synthesis require a membrane-anchored substrate called lipid II, in which the peptidoglycan subunit is linked ...
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