Noriyuki Hatsugai —BIO-PROTOCOL

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Fumiaki Katagiri Dept of Plant and Microbial Biology, University of Minnestoat, USA, USA,
1 protocol

Noriyuki Hatsugai

Research focus

Plant science

1 Author merit
Reviewer merit
Editor merit

1 Protocol published

Quantification of Plant Cell Death by Electrolyte Leakage Assay

Authors: Noriyuki Hatsugai and Fumiaki Katagiri, date: 03/05/2018, view: 16790, Q&A: 2

We describe a protocol to measure the electrolyte leakage from plant tissues, resulting from loss of cell membrane integrity, which is a common definition of cell death. This simple protocol is designed to measure the electrolyte leakage from a ...

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TINA AGARWAL

PURDUE UNIVERSITY

Hello there, I am wondering if the number of leaf disc used has any significance in electrolyte measurement. I am working with cucumber leaves and am using 4 leaf disc per plant in 10 ml DI-water, as this volume helps me to measure the conductivity (we have big electrode like meter (looks like pH meter). Some papers have recommended to use 200 mgs leaf tissue or one leaf disc per sample. So it is bit confusing as to we have to use a certain amount of tissue or as long as it is consistent across the board it should be fine, any suggestions?

5/20/2021 8:41:55 AM Reply

TINA AGARWAL

PURDUE UNIVERSITY

Thank you!
Your response was helpful, I will try to do some trials to figure out a right balance between tissue and water volume.

2021-05-26 12:54:16

Fumiaki Katagiri

Dept of Plant and Microbial Biology, University of Minnestoat, USA, USA,

Dear Tina,
The number of discs, the volume of the well, and such are by no means appropriate in different cases. If your conductivity meter requires a large volume, you would need to put a higher amount of tissue - the sensitivity of the measurement depends on tissue amount/water volume. On the other hand, if you use a larger amount of tissue, the response of a higher number of cells get averaged, which may result in an underestimate of SD in the cell response. You just need to decide the scale of measurement considering the practicality and the potential trade-offs in different experimental system.

2021-05-21 12:13:12