Anne Ephrussi Developmental Biology Unit, European Molecular Biology Laboratory (EMBL), Germany
2 protocols

Imre Gáspár Developmental Biology Unit, European Molecular Biology Laboratory (EMBL), Germany
2 protocols

Frank Wippich
  • Developmental Biology Unit, European Molecular Biology Laboratory (EMBL), 69117 Germany, 69117 Germany,
  • 1 Author merit

Education

PhD, ETH Zurich, Switzerland, 2013

Current position

PostDoc, Developmental Biology Unit, European Molecular Biology Laboratory, Germany

Publications

  1. Gaspar, I., Wippich, F. and Ephrussi, A. (2017). Enzymatic production of single-molecule FISH and RNA capture probes. RNA 23(10): 1582-1591.
  2. Halstead, J. M., Wilbertz, J. H., Wippich, F., Lionnet, T., Ephrussi, A. and Chao, J. A. (2016). TRICK: A Single-Molecule Method for Imaging the First Round of Translation in Living Cells and Animals. Methods Enzymol 572: 123-157.
  3. Halstead, J. M., Lionnet, T., Wilbertz, J. H., Wippich, F., Ephrussi, A., Singer, R. H. and Chao, J. A. (2015). Translation. An RNA biosensor for imaging the first round of translation from single cells to living animals. Science 347(6228): 1367-1671.
  4. Wippich, F., Bodenmiller, B., Trajkovska, M. G., Wanka, S., Aebersold, R. and Pelkmans, L. (2013). Dual specificity kinase DYRK3 couples stress granule condensation/dissolution to mTORC1 signaling. Cell 152(4): 791-805.
  5. Langhorst, M. F., Reuter, A., Jaeger, F. A., Wippich, F. M., Luxenhofer, G., Plattner, H. and Stuermer, C. A. (2008). Trafficking of the microdomain scaffolding protein reggie-1/flotillin-2. Eur J Cell Biol 87(4): 211-226.
1 Protocol published
Terminal Deoxynucleotidyl Transferase Mediated Production of Labeled Probes for Single-molecule FISH or RNA Capture
Authors:  Imre Gaspar, Frank Wippich and Anne Ephrussi, date: 03/05/2018, view: 2023, Q&A: 0
Arrays of short, singly-labeled ssDNA oligonucleotides enable in situ hybridization with single molecule sensitivity and efficient transcript specific RNA capture. Here, we describe a simple, enzymatic protocol that can be carried out using ...
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