Chiara Ambrogio University of Turin
30 protocols

William Jennings Valentine
  • Research scientist, National Center of Psychiatry and Neurology
Research focus
  • Cell biology


PhD, University of Tennessee , Memphis, 2004


Valentine WJ, Tokuoka SM, Hishikawa D, Kita Y, Shindou H, and Shimizu T. LPAAT3 incorporates docosahexaenoic acid into skeletal muscle cell membranes and is upregulated by PPARδ activation. J Lipid Res. 59(2):184-194, February 2018.

Hishikawa D, Valentine WJ, Iizuka-Hishikawa Y, Shindou H, and Shimizu T. Metabolism and functions of docosahexaenoic acid-containing membrane glycerophospholipids FEBS Lett 591(18):2730-2744, September 2017.

Valentine WJ. Lipid mediators and microRNA in physiological and disease conditions. In: miRNAs and Human Diseases. Yue J, Lu L, editors. Research Signposts; 2012. pp. 223-256.

Kiss GN, Lee SC, Fells JI, Liu J, Valentine WJ, Fujiwara Y, Thompson KE, Yates CR, Sümegi B, Tigyi G. Mitigation of radiation injury by selective stimulation of the LPA(2) receptor. Biochim Biophys Acta 1831(1):117-25, January, 2013.

Valentine WJ, Godwin VI, Osborne DA, Liu J, Fujiwara Y, VanBrocklyn JR, Bittman R, Parrill AL, Tigyi G. FTY720 (Gilenya) phosphate selectivity of sphingosine 1-phosphate receptor subtype 1 (S1P1) G protein-coupled receptor requires motifs in intracellular loop 1 and transmembrane domain 2. J Biol Chem 286(35):30513-25, September, 2011.

Valentine WJ and Tigyi G. High throuput assays to measure intracellular Ca2+ mobilization in cells that express recombinant S1P receptor subtypes. Methods Mol Biol 874:77-87, April, 2012.

Valentine WJ*, Kiss GN*, E S, Liu J, E Shuyu, Gotoh M, Murakami-Murofushi K, Pham TC, Baker DL, Lu X, Sun C, Bittman R, Pyne NJ, Tigyi G. (S)-FTY720-vinylphosphonate, an analogue of the immunosuppressive agent FTY720, signals as a pan-antagonist of sphingosine 1-phosphate GPCR and inhibits autotaxin activity. Cell Signal. 2010 Oct;22(10):1543-53. (*equal contributors)

Lu X, Sun C, Valentine WJ, E S, Liu J, Tigyi G, Bittman R. Chiral vinylphosphonate and phosphonate analogues of the immunosuppressive agent FTY720. J Org Chem 74(8):3192-5, Apr, 2009.

Khandoga AL, Fujiwara Y, Goyal P, Pandey D, Tsukahara R, Bolen A, Guo H, Wilke N, Liu J, Valentine WJ, Durgam GG, Miller DD, Jiang G, Prestwich GD, Tigyi G, Siess W. Lysophosphatidic acid-induced platelet shape change revealed through LPA(1-5) receptor-selective probes and albumin. Platelets 19(6):415-27, September, 2008.

Valentine WJ, Fells J, Perygin D, Mujahid S, Yokoyama K, Fujiwara Y, Tsukahara R, VanBrocklyn JR, Parrill AL, Tigyi G. Subtype specific residues involved in ligand activation of the EDG-family lysophosphatidic acid receptors. J Biol Chem 283(18):12175-87, May, 2008.

Valentine WJ, Fujiwara Y, Tsukahara R, Tigyi G. Lysophospholipid signaling: beyond the EDGs. Biochim Biophys Acta 1780(3):597-605, Mar, 2008.
4 Protocols reviewed
Site-specific Labeling of B Cell Receptor and Soluble Immunoglobulin
Authors:  Yu Wang, Zhixun Shen, Zhengpeng Wan and Wanli Liu, date: 09/20/2020, view: 458, Q&A: 0
B lymphocyte activation is regulated by its membrane-bound B cell receptors (BCRs) upon recognizing diverse antigens. It is hypothesized that antigen binding would trigger conformational changes within BCRs, followed by a series of downstream ...
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Colorimetric RhoB GTPase Activity Assay
Authors:  Kossay Zaoui and Stephanie Duhamel, date: 05/05/2020, view: 1568, Q&A: 0
The Ras homologous protein (Rho) GTPase subfamily, including RhoA, RhoB, and RhoC are small molecules (~21 kDa) that act as molecular switches in a wide range of signaling pathways to orchestrate biological processes associated with both ...
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