As regarding for protocol of isolation of stem cell from cord tissue by mixed explant and enzymatic method (trypsin ).,Iam work by this protocol yield many many cells and doing sub culture to passage 6 but when do characterization by flow cytometry (BD accuri) Iam found CD 45 N(range from 70- 90% ) but all positive marker on cell is low(CD 105 range from 20-35%),(CD 70 range from 33- 45%), (CD 146 10-19%).I dont know why a positive marker is low in comparison to negative marker. thank you for good teem bioprotocol.
11/30/2018 11:10:28 AM Reply