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Ali Mortazavi
Department of Developmental and Cell Biology, University of California, USA, USA,
1 protocol
Adler Dillman
Nematology, UC Riverside, USA
2 protocols
Rabi Murad
Department of Developmental and Cell Biology, University of California, USA, USA,
1 protocol
Katherine Williams
Department of Developmental and Cell Biology, University of California, USA, USA,
1 protocol
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Dihong Lu
Department of Nematology, University of California, USA, USA,
Research focus
Systems biology
Contributions
1
Author merit
Reviewer merit
Editor merit
1 Protocol published
Adapting the Smart-seq2 Protocol for Robust Single Worm RNA-seq
Authors:
Lorrayne Serra
,
Dennis Z. Chang
,
Marissa Macchietto
,
Katherine Williams
,
Rabi Murad
,
Dihong Lu
,
Adler R. Dillman
and
Ali Mortazavi
,
date:
02/20/2018,
view:
14210,
Q&A:
3
Most nematodes are small worms that lack enough RNA for regular RNA-seq protocols without pooling hundred to thousand of individuals. We have adapted the Smart-seq2 protocol in order to sequence the transcriptome of an individual worm. While ...
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Pierre-Yves Véronneau
Agriculture & Agri-Food Canada
Hi,
Is there any other reason why you didn't use Illumina barcoded indexes beside the price?
Thanks,
PY
1/21/2019 10:28:34 AM
Reply
Ali Mortazavi
Department of Developmental and Cell Biology, University of California, USA, USA,
The reason I didn’t use Illumina barcodes is because of price.
2019-01-21 16:01:16
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