Dihong Lu —BIO-PROTOCOL

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Ali Mortazavi Department of Developmental and Cell Biology, University of California, USA, USA,
1 protocol

Adler Dillman Nematology, UC Riverside, USA
2 protocols

Rabi Murad Department of Developmental and Cell Biology, University of California, USA, USA,
1 protocol

Katherine Williams Department of Developmental and Cell Biology, University of California, USA, USA,
1 protocol

Dihong Lu

Department of Nematology, University of California, USA, USA,

1 Author merit
Reviewer merit
Editor merit

1 Protocol published

Adapting the Smart-seq2 Protocol for Robust Single Worm RNA-seq

Authors: Lorrayne Serra, Dennis Z. Chang, Marissa Macchietto, Katherine Williams, Rabi Murad, Dihong Lu, Adler R. Dillman and Ali Mortazavi, date: 02/20/2018, view: 3323, Q&A: 2

Most nematodes are small worms that lack enough RNA for regular RNA-seq protocols without pooling hundred to thousand of individuals. We have adapted the Smart-seq2 protocol in order to sequence the transcriptome of an individual worm. While ...

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Pierre-Yves Véronneau

Hi,
I can't find what is the PE buffer used at step F.14
Thanks for your help,
PY

12/18/2018 6:27:12 AM Reply

Pierre-Yves Véronneau

I ordered the QIAquick PCR Purification Kit yesterday and I didn't received it yet. Now I understand that it is part of the kit.
Thanks!

2018-12-18 10:42:58

Lorrayne Serra

Department of Developmental and Cell Biology, University of California, USA, USA,

You can either get a QIAquick PCR Purification Kit -Cat No./ID: 28104 or you can get the concentrate -Cat No./ID: 19065
Buffer PE (concentrate, 100 ml).

2018-12-18 10:19:02