L. Sibley Washington University in St Louis, USA
2 protocols

Shaojun Long Molecular Microbiology, Washington University in St Louis, USA
2 protocols

Kevin M Brown
  • Molecular Microbiology, Washington University in St Louis, USA
  • 2 Author merit
2 Protocols published
CRISPR-mediated Tagging with BirA Allows Proximity Labeling in Toxoplasma gondii
Authors:  Shaojun Long, Kevin M. Brown and L. David Sibley, date: 03/20/2018, view: 2516, Q&A: 0
Defining protein interaction networks can provide key insights into how protein complexes govern complex biological problems. Here we define a method for proximity based labeling using permissive biotin ligase to define protein networks in the ...
Conditional Knockdown of Proteins Using Auxin-inducible Degron (AID) Fusions in Toxoplasma gondii
Authors:  Kevin M. Brown, Shaojun Long and L. David Sibley, date: 02/20/2018, view: 3450, Q&A: 0
Toxoplasma gondii is a member of the deadly phylum of protozoan parasites called Apicomplexa. As a model apicomplexan, there is a great wealth of information regarding T. gondii’s 8,000+ protein coding genes including sequence ...
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