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Coauthors
Joshua Arribere MCD Biology, University of California Santa Cruz, United States, United States,
1 protocol

Andrew Fire Pathology, Stanford University School of Medicine, United States, United States,
1 protocol

Antonio Amat Genetics and Microbiology, Universidad de Murcia, Spain, Spain,
1 protocol

Michael Stadler Molecular and Cell Biology, University of California Berkeley, United States, United States,
1 protocol

Becky Xu Hua Fu
  • Genetics, Stanford University, United States, United States,
  • 1 Author merit

Education

Ph.D., Stanford School of Medicine, USA, 2017

Current position

Post-doc, Pathology, Stanford School of Medicine, USA

Publications

  1. Fu, B. X., Wainberg, M., Kundaje, A. and Fire, A. Z. (2017). High-Throughput Characterization of Cascade type I-E CRISPR Guide Efficacy Reveals Unexpected PAM Diversity and Target Sequence Preferences. Genetics 206(4): 1727-1738.
  2. Fu, B. X., St Onge, R. P., Fire, A. Z. and Smith, J. D. (2016). Distinct patterns of Cas9 mismatch tolerance in vitro and in vivo. Nucleic Acids Res 44(11): 5365-5377.
  3. Bell, R. T., Fu, B. X. and Fire, A. Z. (2016). Cas9 Variants Expand the Target Repertoire in Caenorhabditis elegans. Genetics 202(2): 381-388.
  4. Meyer, D., Fu, B. X. and Heyer, W. D. (2015). DNA polymerases delta and lambda cooperate in repairing double-strand breaks by microhomology-mediated end-joining in Saccharomyces cerevisiae. Proc Natl Acad Sci U S A 112(50): E6907-6916.
  5. Fu, B. X., Hansen, L. L., Artiles, K. L., Nonet, M. L. and Fire, A. Z. (2014). Landscape of target:guide homology effects on Cas9-mediated cleavage. Nucleic Acids Res 42(22): 13778-13787.
  6. Arribere, J. A., Bell, R. T., Fu, B. X., Artiles, K. L., Hartman, P. S. and Fire, A. Z. (2014). Efficient marker-free recovery of custom genetic modifications with CRISPR/Cas9 in Caenorhabditis elegans. Genetics 198(3): 837-846.
  7. Liddell, L. C., Manthey, G. M., Owens, S. N., Fu, B. X. and Bailis, A. M. (2013). Alleles of the homologous recombination gene, RAD59, identify multiple responses to disrupted DNA replication in Saccharomyces cerevisiae. BMC Microbiol 13: 229.
  8. Pannunzio, N. R., Manthey, G. M., Liddell, L. C., Fu, B. X., Roberts, C. M. and Bailis, A. M. (2012). Rad59 regulates association of Rad52 with DNA double-strand breaks. Microbiologyopen 1(3): 285-297.
1 Protocol published
A Small RNA Isolation and Sequencing Protocol and Its Application to Assay CRISPR RNA Biogenesis in Bacteria
Next generation high-throughput sequencing has enabled sensitive and unambiguous analysis of RNA populations in cells. Here, we describe a method for isolation and strand-specific sequencing of small RNA pools from bacteria that can be multiplexed ...