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Coauthors
Daan Swarts Department of Biochemistry, University of Zurich, Switzerland
3 protocols

Martin Jinek 1 protocol

John Oost Department of Agrotechnology and Food Sciences, Wageningen University, the Netherlands, the Netherlands,
3 protocols

P Mohanraju
  • Wageningen University and Research
Research focus
  • Microbiology
  • 1 Author merit
1 Protocol published
Heterologous Expression and Purification of the CRISPR-Cas12a/Cpf1 Protein
Authors:  Prarthana Mohanraju, John van der Oost, Martin Jinek and Daan C. Swarts, date: 05/05/2018, view: 1413, Q&A: 0
This protocol provides step by step instructions (Figure 1) for heterologous expression of Francisella novicida Cas12a (previously known as Cpf1) in Escherichia coli. It additionally includes a protocol for high-purity purification ...