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Nicolaza Pariona Mendoza
  • Institute of Ecology
Research focus
  • Environmental Nanotechnology
2 Protocols feedback
MTT Assay for Cytotoxicity Assessment in Oryza sativa Root Tissue
Authors:  Snehalata Majumdar, Titir Guha and Rita Kundu, date: 11/20/2017, view: 1319, Q&A: 0
Cytotoxicity of different compounds are commonly evaluated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. This assay is mainly used to study cell viability in cell lines (Carmichael et al., 1987). In this study, the protocol is being used to determine the cell viability of plant roots, treated with different ...
Measurement of Endogenous H2O2 and NO and Cell Viability by Confocal Laser Scanning Microscopy
Authors:  Mi-Mi Wu, Xian-Ge Ma and Jun-Min He, date: 10/05/2013, view: 6997, Q&A: 1
Recently, there is compelling evidence that hydrogen peroxide (H2O2) and nitric oxide (NO) function as signaling molecules in plants, mediating a range of responses including stomatal movement. Thus, the choice of sensitive methods for detection of endogenous H2O2 and NO in guard cells are very important ...