Takehito Inaba Department of Agricultural and Environmental Sciences, Faculty of Agriculture, University of Miyazaki, Japan, Japan,
1 protocol

Susumu Uehara Department of Agricultural and Environmental Sciences, Faculty of Agriculture, University of Miyazaki, Japan, Japan,
1 protocol

Yasuko Ito-Inaba
  • Department of Agricultural and Environmental Sciences, Faculty of Agriculture, University of Miyazaki, Japan, Japan,
  • 1 Author merit

Education

Ph.D.(Agriculture), The University of Tokyo, Japan

Current position

Associate Professor, Agriculture, University of Miyazaki, Japan

Publications

  1. Tokumaru, M., Adachi, F., Toda, M., Ito-Inaba, Y., Yazu, F., Hirosawa, Y., Sakakibara, Y., Suiko, M., Kakizaki, T. and Inaba, T. (2017). Ubiquitin-Proteasome Dependent Regulation of the GOLDEN2-LIKE 1 Transcription Factor in Response to Plastid Signals. Plant Physiol 173(1): 524-535.
  2. Ito-Inaba, Y., Masuko-Suzuki, H., Maekawa, H., Watanabe, M. and Inaba, T. (2016). Characterization of two PEBP genes, SrFT and SrMFT, in thermogenic skunk cabbage (Symplocarpus renifolius). Sci Rep 6: 29440.
  3. Uehara, S., Adachi, F., Ito-Inaba, Y. and Inaba, T. (2016). Specific and Efficient Targeting of Cyanobacterial Bicarbonate Transporters to the Inner Envelope Membrane of Chloroplasts in Arabidopsis. Front Plant Sci 7: 16.
  4. Ito-Inaba, Y. (2014). Thermogenesis in skunk cabbage (Symplocarpus renifolius): New insights from the ultrastructure and gene expression profiles. Advances in Horticultural Science 28: 73-78.
  5. Okawa, K., Inoue, H., Adachi, F., Nakayama, K., Ito-Inaba, Y., Schnell, D. J., Uehara, S. and Inaba, T. (2014). Targeting of a polytopic membrane protein to the inner envelope membrane of chloroplasts in vivo involves multiple transmembrane segments. J Exp Bot 65(18): 5257-5265.
  6. Ito-Inaba, Y., Masuko, H., Watanabe, M. and Inaba, T. (2012). Isolation and gene expression analysis of a papain-type cysteine protease in thermogenic skunk cabbage (Symplocarpus renifolius). Biosci Biotechnol Biochem 76(10): 1990-1992.
  7. Ito-Inaba, Y., Hida, Y., Matsumura, H., Masuko, H., Yazu, F., Terauchi, R., Watanabe, M. and Inaba, T. (2012). The gene expression landscape of thermogenic skunk cabbage suggests critical roles for mitochondrial and vacuolar metabolic pathways in the regulation of thermogenesis. Plant Cell Environ 35(3): 554-566.
  8. Ito-Inaba, Y., Hida, Y. and Inaba, T. (2009). What is critical for plant thermogenesis? Differences in mitochondrial activity and protein expression between thermogenic and non-thermogenic skunk cabbages. Planta 231(1): 121-130.
  9. Ito-Inaba, Y., Sato, M., Masuko, H., Hida, Y., Toyooka, K., Watanabe, M. and Inaba, T. (2009). Developmental changes and organelle biogenesis in the reproductive organs of thermogenic skunk cabbage (Symplocarpus renifolius). J Exp Bot 60(13): 3909-3922.
  10. Ito-Inaba, Y., Hida, Y., Mori, H. and Inaba, T. (2008). Molecular identity of uncoupling proteins in thermogenic skunk cabbage. Plant Cell Physiol 49(12): 1911-1916.
  11. Ito-Inaba, Y., Hida, Y., Ichikawa, M., Kato, Y. and Yamashita, T. (2008). Characterization of the plant uncoupling protein, SrUCPA, expressed in spadix mitochondria of the thermogenic skunk cabbage. J Exp Bot 59(4): 995-1005.
  12. Ito, Y., Kanamaru, K., Taniguchi, N., Miyamoto, S. and Tokuda, H. (2006). A novel ligand bound ABC transporter, LolCDE, provides insights into the molecular mechanisms underlying membrane detachment of bacterial lipoproteins. Mol Microbiol 62(4): 1064-1075.
  13. Ito, Y., Matsuzawa, H., Matsuyama, S., Narita, S. and Tokuda, H. (2006). Genetic analysis of the mode of interplay between an ATPase subunit and membrane subunits of the lipoprotein-releasing ATP-binding cassette transporter LolCDE. J Bacteriol 188(8): 2856-2864.
  14. Ito, Y., Tomita, T., Roy, N., Nakano, A., Sugawara-Tomita, N., Watanabe, S., Okai, N., Abe, N. and Kamio, Y. (2003). Cloning, Expression, and Cell Surface Localization of Paenibacillus sp. Strain W-61 Xylanase 5, a Multidomain Xylanase. Applied and Environmental Microbiology 69: 6969-78.
1 Protocol published
Investigating Localization of Chimeric Transporter Proteins within Chloroplasts of Arabidopsis thaliana
Authors:  Susumu Uehara, Yasuko Ito-Inaba and Takehito Inaba, date: 02/05/2018, view: 1774, Q&A: 0
In this protocol, we describe a method to design chimeric proteins for specific targeting to the inner envelope membrane (IEM) of Arabidopsis chloroplasts and the confirmation of their localization by biochemical analysis. Specific ...
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