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Coauthors
Karolin Luger Howard Hughes Medical Institute at University of Colorado Boulder, USA
2 protocols

Francesca Mattiroli Hubrecht Institute
2 protocols

Yajie Gu
  • Howard Hughes Medical Institute at University of Colorado Boulder, USA
  • 2 Author merit

Education

Ph.D, Colorado State University, United States, 2018

Current position

Graduate, Biochemistry, colorado state university, United States

Publications

  1. Mattiroli, F., Gu, Y., Yadav, T., Balsbaugh, J. L., Harris, M. R., Findlay, E. S., Liu, Y., Radebaugh, C. A., Stargell, L. A., Ahn, N. G., Whitehouse, I. and Luger, K. (2017). DNA-mediated association of two histone-bound complexes of yeast Chromatin Assembly Factor-1 (CAF-1) drives tetrasome assembly in the wake of DNA replication. Elife 6.
  2. Mattiroli, F., Gu, Y., Balsbaugh, J. L., Ahn, N. G. and Luger, K. (2017). The Cac2 subunit is essential for productive histone binding and nucleosome assembly in CAF-1. Sci Rep 7: 46274.
  3. Muthurajan, U., Mattiroli, F., Bergeron, S., Zhou, K., Gu, Y., Chakravarthy, S., Dyer, P., Irving, T. and Luger, K. (2016). In Vitro Chromatin Assembly: Strategies and Quality Control. Methods Enzymol 573: 3-41.
2 Protocols published
Measuring Nucleosome Assembly Activity in vitro with the Nucleosome Assembly and Quantification (NAQ) Assay
Authors:  Francesca Mattiroli, Yajie Gu and Karolin Luger, date: 02/05/2018, view: 743, Q&A: 0
Nucleosomes organize the eukaryotic genome into chromatin. In cells, nucleosome assembly relies on the activity of histone chaperones, proteins with high binding affinity to histones. At least a subset of histone chaperones promotes histone ...
FRET-based Stoichiometry Measurements of Protein Complexes in vitro
Authors:  Francesca Mattiroli, Yajie Gu and Karolin Luger, date: 02/05/2018, view: 818, Q&A: 0
For a complete understanding of biochemical reactions, information on complex stoichiometry is essential. However, measuring stoichiometry is experimentally challenging. Our lab has developed a FRET-based assay to study protein complex stoichiometry ...