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A peer-reviewed protocol journal. No publication fee; no access fee.
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Alessandro Didonna
University of California, San Francisco
27 protocols
Michela Perego
The Wistar Institute
3 protocols
Tomas Aparicio
Columbia University
7 protocols
Zinan Zhou
Boston Children’s Hospital
16 protocols
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Reviewer
Silvia Caggia
Post-Doc, CLARK ATLANTA UNIVERSITY
Research focus
Cancer biology
prostate cancer, TFG-beta signaling pathway, GPCR signaling, migration and metastasis
Contributions
Reviewer merit
Editor merit
Education
PhD in Oncology, University of Catania, 2012
Lab information
Shafiq Khan’s laboratory
2 Protocols reviewed
Preserve Cultured Cell Cytonemes through a Modified Electron Microscopy Fixation
Authors:
Eric T. Hall
and
Stacey K. Ogden
,
date:
07/05/2018,
view:
2347,
Q&A:
0
Immunocytochemistry of cultured cells is a common and effective technique for determining compositions and localizations of proteins within cellular structures. However, traditional cultured cell fixation and staining protocols are not effective in ...
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An Image-based Assay for High-throughput Analysis of Cell Proliferation and Cell Death of Adherent Cells
Authors:
Paula Szalai
and
Nikolai Engedal
,
date:
05/05/2018,
view:
3951,
Q&A:
0
In this protocol, we describe a method to monitor cell proliferation and death by live-cell imaging of propidium iodide (PI)-stained adherent mammalian cells. PI is widely used to assess cell death. However, it is usually used in end-point assays. ...
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