Tanneke den Blaauwen Bacterial Cell Biology & Physiology, University of Amsterdam, The Netherlands, The Netherlands,
2 protocols

Svetlana Alexeeva Laboratory of Food Microbiology, Wageningen University, The Netherlands, The Netherlands,
1 protocol

Nils Meiresonne Bacterial Cell Biology & Physiology, University of Amsterdam, The Netherlands, The Netherlands,
1 protocol

René van der Ploeg
  • Hogeschool Utrecht, Hogeschool Utrecht, The Netherlands, The Netherlands,
  • 1 Author merit


PhD, University of Groningen, The Netherlands, 2009

Current position

Lecturer, Life Sciences, HU "University of Applied Sciences", The Netherlands


  1. Meiresonne, N. Y., van der Ploeg, R., Hink, M. A. and den Blaauwen, T. (2017). Activity-Related Conformational Changes in d,d-Carboxypeptidases Revealed by In Vivo Periplasmic Forster Resonance Energy Transfer Assay in Escherichia coli. MBio 8(5).
  2. van der Ploeg, R., Goudelis, S. T. and den Blaauwen, T. (2015). Validation of FRET Assay for the Screening of Growth Inhibitors of Escherichia coli Reveals Elongasome Assembly Dynamics. Int J Mol Sci 16(8): 17637-17654.
  3. van der Ploeg, R., Verheul, J., Vischer, N. O., Alexeeva, S., Hoogendoorn, E., Postma, M., Banzhaf, M., Vollmer, W. and den Blaauwen, T. (2013). Colocalization and interaction between elongasome and divisome during a preparative cell division phase in Escherichia coli. Mol Microbiol 87(5): 1074-1087.
  4. Goosens, V. J., Otto, A., Glasner, C., Monteferrante, C. C., van der Ploeg, R., Hecker, M., Becher, D. and van Dijl, J. M. (2013). Novel twin-arginine translocation pathway-dependent phenotypes of Bacillus subtilis unveiled by quantitative proteomics. J Proteome Res 12(2): 796-807.
  5. van der Ploeg, R., Monteferrante, C. G., Piersma, S., Barnett, J. P., Kouwen, T. R., Robinson, C. and van Dijl, J. M. (2012). High-salinity growth conditions promote Tat-independent secretion of Tat substrates in Bacillus subtilis. Appl Environ Microbiol 78(21): 7733-7744.
  6. Monteferrante, C. G., Miethke, M., van der Ploeg, R., Glasner, C. and van Dijl, J. M. (2012). Specific targeting of the metallophosphoesterase YkuE to the bacillus cell wall requires the twin-arginine translocation system. J Biol Chem 287(35): 29789-29800.
  7. Van der Ploeg, R. and den Blaauwen, T. (2012). In vivo bacterial morphogenetic protein interactions. In: Meghea, A. (Ed). Molecular Interaction, publisher Intech. pp: 229-262, Rijeka.
  8. van der Ploeg, R., Barnett, J. P., Vasisht, N., Goosens, V. J., Pother, D. C., Robinson, C. and van Dijl, J. M. (2011). Salt sensitivity of minimal twin arginine translocases. J Biol Chem 286(51): 43759-43770.
  9. van der Ploeg, R., Mader, U., Homuth, G., Schaffer, M., Denham, E. L., Monteferrante, C. G., Miethke, M., Marahiel, M. A., Harwood, C. R., Winter, T., Hecker, M., Antelmann, H. and van Dijl, J. M. (2011). Environmental salinity determines the specificity and need for Tat-dependent secretion of the YwbN protein in Bacillus subtilis. PLoS One 6(3): e18140.
  10. Fraipont, C., Alexeeva, S., Wolf, B., van der Ploeg, R., Schloesser, M., den Blaauwen, T. and Nguyen-Disteche, M. (2011). The integral membrane FtsW protein and peptidoglycan synthase PBP3 form a subcomplex in Escherichia coli. Microbiology 157(Pt 1): 251-259.
  11. Barnett, J. P., van der Ploeg, R., Eijlander, R. T., Nenninger, A., Mendel, S., Rozeboom, R., Kuipers, O. P., van Dijl, J. M. and Robinson, C. (2009). The twin-arginine translocation (Tat) systems from Bacillus subtilis display a conserved mode of complex organization and similar substrate recognition requirements. FEBS J 276(1): 232-243.
  12. Kolkman, M. A., van der Ploeg, R., Bertels, M., van Dijk, M., van der Laan, J., van Dijl, J. M. and Ferrari, E. (2008). The twin-arginine signal peptide of Bacillus subtilis YwbN can direct either Tat- or Sec-dependent secretion of different cargo proteins: secretion of active subtilisin via the B. subtilis Tat pathway. Appl Environ Microbiol 74(24): 7507-7513.
  13. Kouwen, T. R., Antelmann, H., van der Ploeg, R., Denham, E. L., Hecker, M. and van Dijl, J. M. (2009). MscL of Bacillus subtilis prevents selective release of cytoplasmic proteins in a hypotonic environment. Proteomics 9(4): 1033-1043.
  14. Kouwen, T. R., van der Ploeg, R., Antelmann, H., Hecker, M., Homuth, G., Mader, U. and van Dijl, J. M. (2009). Overflow of a hyper-produced secretory protein from the Bacillus Sec pathway into the Tat pathway for protein secretion as revealed by proteogenomics. Proteomics 9(4): 1018-1032.
  15. Jongbloed, J. D., van der Ploeg, R. and van Dijl, J. M. (2006). Bifunctional TatA subunits in minimal Tat protein translocases. Trends Microbiol 14(1): 2-4.
  16. Kamerbeek, N. M., van der Ploeg, R., Olsthoorn, A. J. J., Tahallah, N., Heck, A. J. R., Malito, E., Janssen, D. B. and Fraaije, M. W. (2005). Exploring the role of the N-terminus of 4-hydroxyacetophenone monooxygenase. In: Nishino, T., Miura,  R., Tanokura, M. and Fukui, K. (Eds). Flavins and flavoproteins pp. 173-178.
1 Protocol published
Detection of Protein Interactions in the Cytoplasm and Periplasm of Escherichia coli by Förster Resonance Energy Transfer
This protocol was developed to qualitatively and quantitatively detect protein-protein interactions in Escherichia coli by Förster Resonance Energy Transfer (FRET). The described assay allows for the previously impossible in vivo ...