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Modesto Redrejo-Rodriguez
Biochemistry Department, Madrid Autonomous University
2 protocols
Margarita Salas
Centro de Biología Molecular Severo Ochoa, Centro de Biología Molecular Severo Ochoa, Spain, Spain,
1 protocol
Ana Lechuga
Centro de Biología Molecular Severo Ochoa, Consejo Superior de Investigaciones científicas y Universidad Autónoma de Madrid, Spain, Spain,
1 protocol
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Mónica Berjón-Otero
Present address: Max Planck Institute for Medical Research, Germany, Germany,
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1 Protocol published
Analysis of Direct Interaction between Viral DNA-binding Proteins by Protein Pull-down Co-immunoprecipitation Assay
Authors:
Ana Lechuga
,
Mónica Berjón-Otero
,
Margarita Salas
and
Modesto Redrejo-Rodríguez
,
date:
01/05/2018,
view:
2173,
Q&A:
0
This protocol analyzes the direct interaction between two DNA-binding proteins by pull-down co-immunoprecipitation. One of the proteins is overexpressed in
E. coli
as HA-tagged recombinant protein and cell-free extracts are ...
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