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Sylvain Moineau Université Laval
1 protocol

Ariane Renaud 1 protocol


Geneviève M. Rousseau
  • 1 Author merit

Education

M.Sc in Microbiology, Département de biochimie, de microbiologie, et de bio-informatique, Université Laval, Canada, 2007

Current position

Research assistant, Département de biochimie, de microbiologie, et de bio-informatique, Université Laval, Québec, Canada

Publications

  1. Hynes, A. P., Rousseau, G. M., Lemay, M. L., Horvath, P., Romero, D. A., Fremaux, C. and Moineau, S. (2017). An anti-CRISPR from a virulent streptococcal phage inhibits Streptococcus pyogenes Cas9. Nat Microbiol 2(10): 1374-1380.
  2. Hamdi, S., Rousseau, G. M., Labrie, S. J., Tremblay, D. M., Kourda, R. S., Ben Slama, K. and Moineau, S. (2017). Characterization of two polyvalent phages infecting Enterobacteriaceae. Sci Rep 7: 40349.
  3. Hamdi, S., Rousseau, G. M., Labrie, S. J., Kourda, R. S., Tremblay, D. M., Moineau, S. and Slama, K. B. (2016). Characterization of Five Podoviridae Phages Infecting Citrobacter freundii. Front Microbiol 7: 1023.
  4. Mercanti, D. J., Rousseau, G. M., Capra, M. L., Quiberoni, A., Tremblay, D. M., Labrie, S. J. and Moineau, S. (2015). Genomic Diversity of Phages Infecting Probiotic Strains of Lactobacillus paracasei. Appl Environ Microbiol 82(1): 95-105.
  5. Pujato, S. A., Mercanti, D. J., Guglielmotti, D. M., Rousseau, G. M., Moineau, S., Reinheimer, J. A. and Quiberoni Adel, L. (2015). Phages of dairy Leuconostoc mesenteroides: genomics and factors influencing their adsorption. Int J Food Microbiol 201: 58-65.
  6. Delisle, A. L., Guo, M., Chalmers, N. I., Barcak, G. J., Rousseau, G. M. and Moineau, S. (2012). Biology and genome sequence of Streptococcus mutans phage M102AD. Appl Environ Microbiol 78(7): 2264-2271.
  7. Rousseau, G. M., Deveau, H. and Moineau, S. (2012). Phage Resistance in Lactic Acid Bacteria. In: Quiberoni, A. and Reinheimer, J. (Eds). Bacteriophages in Dairy Processing. Nova Science Publishers p: 149-174.
  8. Verreault, D., Gendron, L., Rousseau, G. M., Veillette, M., Masse, D., Lindsley, W. G., Moineau, S. and Duchaine, C. (2011). Detection of airborne lactococcal bacteriophages in cheese manufacturing plants. Appl Environ Microbiol 77(2): 491-497.
  9. Quiberoni, A., Moineau, S., Rousseau, G. M., Reinheimer, J. and Ackermann, H. W. (2010). Review: Streptococcus thermophilus bacteriophages. Int Dairy J 20: 657-664.
  10. Verreault, D., Rousseau, G. M., Gendron, L., Massé, D., Moineau, S. and Duchaine, C. (2010). Comparison of polycarbonate and polytetrafluoroethylene filters for sampling of airborne phages. Aerosol Sci Technol 44: 203-207.
  11.  Rousseau, G. M. and Moineau, S. (2009). Evolution of Lactococcus lactis phages within a cheese factory. Appl Environ Microbiol 75(16): 5336-5344.
  12. Haaber, J., Rousseau, G. M., Hammer, K. and Moineau, S. (2009). Identification and characterization of a phage gene sav involved in sensitivity to the lactococcal abortive infection mechanism AbiV. Appl Environ Microbiol 75: 2484-2494.
  13. Donovan, D. M., Dong, S., Garrett, W., Rousseau, G. M., Moineau, S. and Pritchard, D. G. (2006). Peptidoglycan hydrolase fusions maintain their parental specificities. Appl Environ Microbiol 72(4): 2988-2996.
  14. Donovan, D. M., Foster-Frey, J., Dong, S., Rousseau, G. M., Moineau, S. and Pritchard, D. G. (2006). The cell lysis activity of the Streptococcus agalactiae bacteriophage B30 endolysin relies on the cysteine, histidine-dependent amidohydrolase/peptidase domain. Appl Environ Microbiol 72(7): 5108-5112.
1 Protocol published
Targeted Genome Editing of Virulent Phages Using CRISPR-Cas9
Authors:  Marie-Laurence Lemay, Ariane C. Renaud, Geneviève M. Rousseau and Sylvain Moineau, date: 01/05/2018, view: 3182, Q&A: 0
This protocol describes a straightforward method to generate specific mutations in the genome of strictly lytic phages. Briefly, a targeting CRISPR-Cas9 system and a repair template suited for homologous recombination are provided inside a bacterial ...