Sylvain Moineau Université Laval
1 protocol

Ariane Renaud 1 protocol

Marie-Laurence Lemay
  • 1 Author merit


B.Sc. Microbiology, Université Laval, Canada, 2014

Current position

Ph.D. Candidate, Département de biochimie, de microbiologie et de bio-informatique, Université Laval, Canada


  1. Hynes, A. P., Rousseau, G. M., Lemay, M. L., Horvath, P., Romero, D., Fremaux, C. and Moineau, S. (2017). An anti-CRISPR from a virulent streptococcal phage inhibits Streptococcus pyogenes Cas9. Nat Microbiol 2: 1374-1380.
  2. Lemay, M. L., Horvath, P. and Moineau, S. (2017). The CRISPR-Cas app goes viral. Curr Opin Microbiol 37: 103-109.
  3. Lemay, M. L., Tremblay, D. M. and Moineau, S. (2017). Genome Engineering of Virulent Lactococcal Phages Using CRISPR-Cas9. ACS Synth Biol 6(7): 1351-1358.
  4. Hynes, A. P., Lemay, M. L., Trudel, L., Deveau, H., Frenette, M., Tremblay, D. M. and Moineau, S. (2017). Detecting natural adaptation of the Streptococcus thermophilus CRISPR-Cas systems in research and classroom settings. Nat Protoc 12(3): 547-565.
  5. Hynes, A. P., Lemay, M. L. and Moineau, S. (2016). Applications of CRISPR-Cas in its natural habitat. Curr Opin Chem Biol 34: 30-36.
  6. Monge-Roffarello, B., Labbe, S. M., Roy, M. C., Lemay, M. L., Coneggo, E., Samson, P., Lanfray, D. and Richard, D. (2014). The PVH as a site of CB1-mediated stimulation of thermogenesis by MC4R agonism in male rats. Endocrinology 155(9): 3448-3458.
1 Protocol published
Targeted Genome Editing of Virulent Phages Using CRISPR-Cas9
Authors:  Marie-Laurence Lemay, Ariane C. Renaud, Geneviève M. Rousseau and Sylvain Moineau, date: 01/05/2018, view: 3188, Q&A: 0
This protocol describes a straightforward method to generate specific mutations in the genome of strictly lytic phages. Briefly, a targeting CRISPR-Cas9 system and a repair template suited for homologous recombination are provided inside a bacterial ...
您可以切换至中文站点,以获得适用于中国用户的内容 中文站点
We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.