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Coauthors
Sylvain Moineau Université Laval
1 protocol

Ariane Renaud 1 protocol

Marie-Laurence Lemay
  • 1 Author merit

Education

B.Sc. Microbiology, Université Laval, Canada, 2014

Current position

Ph.D. Candidate, Département de biochimie, de microbiologie et de bio-informatique, Université Laval, Canada

Publications

  1. Hynes, A. P., Rousseau, G. M., Lemay, M. L., Horvath, P., Romero, D., Fremaux, C. and Moineau, S. (2017). An anti-CRISPR from a virulent streptococcal phage inhibits Streptococcus pyogenes Cas9. Nat Microbiol 2: 1374-1380.
  2. Lemay, M. L., Horvath, P. and Moineau, S. (2017). The CRISPR-Cas app goes viral. Curr Opin Microbiol 37: 103-109.
  3. Lemay, M. L., Tremblay, D. M. and Moineau, S. (2017). Genome Engineering of Virulent Lactococcal Phages Using CRISPR-Cas9. ACS Synth Biol 6(7): 1351-1358.
  4. Hynes, A. P., Lemay, M. L., Trudel, L., Deveau, H., Frenette, M., Tremblay, D. M. and Moineau, S. (2017). Detecting natural adaptation of the Streptococcus thermophilus CRISPR-Cas systems in research and classroom settings. Nat Protoc 12(3): 547-565.
  5. Hynes, A. P., Lemay, M. L. and Moineau, S. (2016). Applications of CRISPR-Cas in its natural habitat. Curr Opin Chem Biol 34: 30-36.
  6. Monge-Roffarello, B., Labbe, S. M., Roy, M. C., Lemay, M. L., Coneggo, E., Samson, P., Lanfray, D. and Richard, D. (2014). The PVH as a site of CB1-mediated stimulation of thermogenesis by MC4R agonism in male rats. Endocrinology 155(9): 3448-3458.
1 Protocol published
Targeted Genome Editing of Virulent Phages Using CRISPR-Cas9
Authors:  Marie-Laurence Lemay, Ariane C. Renaud, Geneviève M. Rousseau and Sylvain Moineau, date: 01/05/2018, view: 1854, Q&A: 0
This protocol describes a straightforward method to generate specific mutations in the genome of strictly lytic phages. Briefly, a targeting CRISPR-Cas9 system and a repair template suited for homologous recombination are provided inside a bacterial ...