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Coauthors
Alexandre David IGF, CNRS, INSERM, Univ. Montpellier, France, France,
2 protocols

Jonathan Yewdell Laboratory of Viral Diseases, National Institute of Allergy and Infectious Diseases, USA , USA ,
2 protocols

Amandine Bastide
  • IGF, CNRS, INSERM, Univ. Montpellier, France, France,
  • 2 Author merit
2 Protocols published
Determining Ribosome Translational Status by Ribo-ELISA
Authors:  Amandine Bastide, Jonathan W. Yewdell and Alexandre David, date: 01/05/2018, view: 1301, Q&A: 0
The Ribo-ELISA was originally developed to elucidate the basis for the ribopuromycylation method (RPM)-based detection of ribosome bound nascent chains. The Ribo-ELISA enables characterization of the translational status of ribosomes, and can be ...
The RiboPuromycylation Method (RPM): an Immunofluorescence Technique to Map Translation Sites at the Sub-cellular Level
Authors:  Amandine Bastide, Jonathan W. Yewdell and Alexandre David, date: 01/05/2018, view: 1589, Q&A: 0
While isotopic labeling of amino acids remains the reference method in the field for quantifying translation rate, it does not provide any information on spatial localization of translation sites. The rationale behind developing the ...